Inactive extracellular superoxide dismutase disrupts secretion and function of active extracellular superoxide dismutase

Byeongwook Jeon, Byung Hak Kim, Yun Sang Lee, Sungsub Kim, Jong Bok Yoon, Tae Yoon Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Extracellular superoxide dismutase (EC-SOD) is an antioxidant enzyme that protects cells and tissues from extracellular damage by eliminating superoxide anion radicals produced during metabolism. Two different forms of EC-SOD exist, and their different enzyme activities are a result of different disulfide bond patterns. Although only two folding variants have been discovered so far, five folding variants are theoretically possible. Therefore, we constructed five different mutant EC-SOD expression vectors by substituting cysteine residues with serine residues and evaluated their expression levels and enzyme activities. The mutant EC-SODs were expressed at lower levels than that of wild-type EC-SOD, and all of the mutants exhibited inhibited extracellular secretion, except for C195S ECSOD. Finally, we demonstrated that co-expression of wild-type EC-SOD and any one of the mutant EC-SODs resulted in reduced secretion of wild-type EC-SOD. We speculate that mutant EC-SOD causes malfunctions in systems such as antioxidant systems and sensitizes tissues to ROS-mediated diseases.

Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalBMB reports
Volume44
Issue number1
DOIs
Publication statusPublished - 2011 Jan

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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