Increased expression of FGF1-mediated signaling molecules in adipose tissue of obese mice

Youngshim Choi, Suhyeon Jang, Myung Sook Choi, Zae Young Ryoo, Taesun Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Fibroblast growth factors (FGFs) are pleiotropic growth factors that control cell proliferation, migration, and differentiation. Herein, we evaluated whether visceral adiposity of mice is accompanied by the alteration of signaling molecules mediated by fibroblast growth factor receptor 1 (FGFR1) induced by using two different male C57BL/6J mice models of obesity namely high-fat diet (HFD)-induced obesity for 12 weeks or mice with genetic deletion of leptin (ob/ob). Both HFD-fed and ob/ob mice exhibited significantly higher messenger RNA (mRNA) levels of FGF1, cyclin D (cycD), transcription factor E2F1, peroxisome proliferator-activated receptor-gamma 2 (PPAR-γ2), CCAAT-enhancer-binding protein alpha (C/EBPα), and adipocyte protein 2 (aP2) genes in their epididymal adipose tissues compared to those of the normal diet (ND)-fed and lean control mice, respectively. In addition, immunoblot analyses of the epididymal adipose tissues revealed that both mice exposed to HFD and ob/ob mice exhibited elevated phosphorylation of FGFR1, extracellular-signal-regulated kinase (ERK), and retinoblastoma (Rb) proteins. These data support the notion that FGF1-mediated signaling represents an important signaling cascade related to adipogenesis, at least partially, among other known signaling pathways. These new findings regarding the molecular mechanisms controlling adipose tissue plasticity provide a novel insight about the functional network with potential therapeutic application against obesity.

Original languageEnglish
Pages (from-to)157-167
Number of pages11
JournalJournal of Physiology and Biochemistry
Volume72
Issue number2
DOIs
Publication statusPublished - 2016 Jun 1

Fingerprint

Fibroblast Growth Factor 1
Obese Mice
Nutrition
Adipose Tissue
Receptor, Fibroblast Growth Factor, Type 1
Tissue
Molecules
Fats
High Fat Diet
E2F1 Transcription Factor
Obesity
CCAAT-Enhancer-Binding Protein-alpha
Cyclin D
Retinoblastoma Protein
Phosphorylation
Fibroblast Growth Factors
PPAR gamma
Extracellular Signal-Regulated MAP Kinases
Cell proliferation
Leptin

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry

Cite this

Choi, Youngshim ; Jang, Suhyeon ; Choi, Myung Sook ; Ryoo, Zae Young ; Park, Taesun. / Increased expression of FGF1-mediated signaling molecules in adipose tissue of obese mice. In: Journal of Physiology and Biochemistry. 2016 ; Vol. 72, No. 2. pp. 157-167.
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Increased expression of FGF1-mediated signaling molecules in adipose tissue of obese mice. / Choi, Youngshim; Jang, Suhyeon; Choi, Myung Sook; Ryoo, Zae Young; Park, Taesun.

In: Journal of Physiology and Biochemistry, Vol. 72, No. 2, 01.06.2016, p. 157-167.

Research output: Contribution to journalArticle

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