Increased expression of phospholipase D1 in the brains of scrapie-infected mice

Jae Kwang Jin, Nam Ho Kim, Do Sik Min, Jae Il Kim, Jin Kyu Choi, Byung Hoon Jeong, Seung Il Choi, Eun Kyoung Choi, Richard I. Carp, Yong Sun Kim

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17 Citations (Scopus)

Abstract

Mitochondrial dysfunction and free radical-induced oxidative damage are critical factors in the pathogenesis of neurodegenerative diseases. Recently, phospholipid breakdown by phospholipase D (PLD) has been recognized as an important signalling pathway in the nervous system. Here, we examined the expression of PLD and alteration of membrane phospholipid in scrapie brain. We have found that protein expression and enzyme activity of PLD1 were increased in scrapie brains compared with controls; in particular, there was an increase in the mitochondrial fraction. PLD1 in mitochondrial membranes from scrapie brains, but not from control brains, was tyrosine phosphorylated. Furthermore, the concentration of mitochondrial phospholipids such as phosphatidylcholine and phosphatidylethanolamine was increased and the content of phosphatidic acid, a product of PLD activity, was up-regulated in the mitochondrial membrane fractions. Immunohistochemically, PLD1 immunoreactivity was significantly increased in activated astrocytes in both cerebral cortex and hippocampus of scrapie brains. Taken together, these results suggest that PLD activation might induce alterations in mitochondrial lipids and, in turn, mediate mitochondrial dysfunction in the brains of scrapie-infected mice.

Original languageEnglish
Pages (from-to)452-461
Number of pages10
JournalJournal of Neurochemistry
Volume92
Issue number3
DOIs
Publication statusPublished - 2005 Feb 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Jin, J. K., Kim, N. H., Min, D. S., Kim, J. I., Choi, J. K., Jeong, B. H., Choi, S. I., Choi, E. K., Carp, R. I., & Kim, Y. S. (2005). Increased expression of phospholipase D1 in the brains of scrapie-infected mice. Journal of Neurochemistry, 92(3), 452-461. https://doi.org/10.1111/j.1471-4159.2004.02881.x