Increased expression of WNK3 in dispersed granule cells in hippocampal sclerosis of mesial temporal lobe epilepsy patients

Kyoung Hoon Jeong, SeHoon Kim, Yun Ho Choi, Inja Cho, Won Joo Kim

Research output: Contribution to journalArticle

Abstract

Granule cell dispersion (GCD) is a common neuropathological feature of hippocampal sclerosis (HS) in patients with temporal lobe epilepsy (TLE). However, the underlying molecular mechanism of GCD formation remains unclear. The present study aimed to investigate the expressional changes of With No Lysine protein kinase subtype 3 (WNK3), a molecule upstream of cation-chloride cotransporters with reciprocal expression in sclerosed hippocampus of TLE patients. Using immunofluorescence staining, we analyzed WNK3 immunoreactivity in hippocampal specimens from histologically normal controls and TLE patients with HS. Our results showed that WNK3 expression was significantly increased in dispersed granule neurons in hippocampal tissues from patients with TLE compared with histologically normal hippocampus. These findings demonstrate a potential association between an increased expression of WNK3 and GCD formation during the chronic phase of epilepsy. Controlling WNK3 expression may thus be a novel therapeutic target in epileptogenesis.

Original languageEnglish
Pages (from-to)58-61
Number of pages4
JournalEpilepsy Research
Volume147
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Temporal Lobe Epilepsy
Sclerosis
Hippocampus
Protein Kinases
Lysine
Fluorescent Antibody Technique
Cations
Chlorides
Epilepsy
Staining and Labeling
Neurons
Therapeutics

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

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abstract = "Granule cell dispersion (GCD) is a common neuropathological feature of hippocampal sclerosis (HS) in patients with temporal lobe epilepsy (TLE). However, the underlying molecular mechanism of GCD formation remains unclear. The present study aimed to investigate the expressional changes of With No Lysine protein kinase subtype 3 (WNK3), a molecule upstream of cation-chloride cotransporters with reciprocal expression in sclerosed hippocampus of TLE patients. Using immunofluorescence staining, we analyzed WNK3 immunoreactivity in hippocampal specimens from histologically normal controls and TLE patients with HS. Our results showed that WNK3 expression was significantly increased in dispersed granule neurons in hippocampal tissues from patients with TLE compared with histologically normal hippocampus. These findings demonstrate a potential association between an increased expression of WNK3 and GCD formation during the chronic phase of epilepsy. Controlling WNK3 expression may thus be a novel therapeutic target in epileptogenesis.",
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Increased expression of WNK3 in dispersed granule cells in hippocampal sclerosis of mesial temporal lobe epilepsy patients. / Jeong, Kyoung Hoon; Kim, SeHoon; Choi, Yun Ho; Cho, Inja; Kim, Won Joo.

In: Epilepsy Research, Vol. 147, 01.11.2018, p. 58-61.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Jeong, Kyoung Hoon

AU - Kim, SeHoon

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AU - Cho, Inja

AU - Kim, Won Joo

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