TY - JOUR
T1 - Increased stability in plasma and enhanced cellular uptake of thermally denatured albumin-coated liposomes
AU - Jung, Suk Hyun
AU - Kim, Sung Kyu
AU - Jung, Soon Hwa
AU - Kim, Eun Hye
AU - Cho, Sun Hang
AU - Jeong, Kyu Sung
AU - Seong, Hasoo
AU - Shin, Byung Cheol
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/4/1
Y1 - 2010/4/1
N2 - Liposomes are nano-scale vesicles that can be used as one of drug carriers. The liposomes are, however, plagued by rapid opsonization of them and hence making their circulation time in bloodstream to be shortened. In this study, cationically charged liposomes of which surface was modified with bovine serum albumin (BSA) were prepared by using electrostatic interaction between cationic liposomes and anionically charged BSA molecules at higher pH than isoelectric point (pI) of BSA. The BSA-coated liposomes (BLs) were denatured by thermal treatment of BL at 100 °C. The thermally denatured BSA-coated liposomes (DBLs) have mean particle diameter of 109 ± 1 nm. Encapsulation of model drug, doxorubicin (DOX), in the liposomes was carried out by using, so called, remote loading method and loading efficiency of DOX in liposomes was about 90%. DBL800 showed higher stability in plasma compared to Doxil®. Results of intracellular uptake evaluated by flow cytometry and confocal microscopy studies showed higher intracellular uptake of DBL800 than that of Doxil®. Consequently, the DBL, of which surface was complexed with denatured protein may be applicable as drug delivery carriers for increasing stability in plasma and enhanced cellular uptake efficacy of anticancer drugs.
AB - Liposomes are nano-scale vesicles that can be used as one of drug carriers. The liposomes are, however, plagued by rapid opsonization of them and hence making their circulation time in bloodstream to be shortened. In this study, cationically charged liposomes of which surface was modified with bovine serum albumin (BSA) were prepared by using electrostatic interaction between cationic liposomes and anionically charged BSA molecules at higher pH than isoelectric point (pI) of BSA. The BSA-coated liposomes (BLs) were denatured by thermal treatment of BL at 100 °C. The thermally denatured BSA-coated liposomes (DBLs) have mean particle diameter of 109 ± 1 nm. Encapsulation of model drug, doxorubicin (DOX), in the liposomes was carried out by using, so called, remote loading method and loading efficiency of DOX in liposomes was about 90%. DBL800 showed higher stability in plasma compared to Doxil®. Results of intracellular uptake evaluated by flow cytometry and confocal microscopy studies showed higher intracellular uptake of DBL800 than that of Doxil®. Consequently, the DBL, of which surface was complexed with denatured protein may be applicable as drug delivery carriers for increasing stability in plasma and enhanced cellular uptake efficacy of anticancer drugs.
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U2 - 10.1016/j.colsurfb.2009.12.002
DO - 10.1016/j.colsurfb.2009.12.002
M3 - Article
C2 - 20036109
AN - SCOPUS:75749128139
VL - 76
SP - 434
EP - 440
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 2
ER -