Increased thrombogenicity in chronic renal failure in a rat model induced by 5/6 ablation/infarction

Tae Jin Song, Il Kwon, Honglim Piao, Jee Eun Lee, Kyeo Rye Han, Yoonkyung Chang, Hyung Jung Oh, Hyun Jung Choi, Kyung Yul Lee, Yong Jae Kim, Ki Hwan Han, Ji Hoe Heo

Research output: Contribution to journalArticle

Abstract

Purpose: Abnormalities in hemostasis and coagulation have been suggested in chronic renal failure (CRF). In this study, we compared processes of thrombus formation between rats with CRF and those with normal kidney function. Materials and Methods: CRF was induced by 5/6 ablation/infarction of the kidneys in Sprague-Dawley rats, and surviving rats after 4 weeks were used. Ferric chloride (FeCl3)-induced thrombosis in the carotid artery was induced to assess thrombus formation. Whole blood clot formation was evaluated using rotational thromboelastometry (ROTEM). Platelet aggregation was assessed with impedance platelet aggregometry. Results: FeCl3-induced thrombus formation was initiated faster in the CRF group than in the control group (13.2±1.1 sec vs. 17.8±1.0 sec, p=0.027). On histological examination, the maximal diameters of thrombi were larger in the CRF group than in the control group (394.2±201.1 μm vs. 114.0±145.1 μm, p=0.039). In extrinsic pathway ROTEM, the CRF group showed faster clot initiation (clotting time, 59.0±7.3 sec vs. 72.8±5.0 sec, p=0.032) and increased clot growth kinetics (α angle, 84.8±0.2° vs. 82.0±0.6°, p=0.008), compared to the control group. Maximal platelet aggregation rate was higher in the CRF group than in the control group (58.2±0.2% vs. 44.6±1.2%, p=0.006). Conclusion: Our study demonstrated that thrombogenicity is increased in rats with CRF. An activated extrinsic coagulation pathway may play an important role in increasing thrombogenicity in CRF.

Original languageEnglish
Pages (from-to)754-759
Number of pages6
JournalYonsei medical journal
Volume59
Issue number6
DOIs
Publication statusPublished - 2018 Aug

Fingerprint

Infarction
Chronic Kidney Failure
Thrombosis
Thrombelastography
Control Groups
Platelet Aggregation
Carotid Artery Thrombosis
Kidney
Hemostasis
Electric Impedance
Sprague Dawley Rats
Blood Platelets
Growth

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Song, Tae Jin ; Kwon, Il ; Piao, Honglim ; Lee, Jee Eun ; Han, Kyeo Rye ; Chang, Yoonkyung ; Oh, Hyung Jung ; Choi, Hyun Jung ; Lee, Kyung Yul ; Kim, Yong Jae ; Han, Ki Hwan ; Heo, Ji Hoe. / Increased thrombogenicity in chronic renal failure in a rat model induced by 5/6 ablation/infarction. In: Yonsei medical journal. 2018 ; Vol. 59, No. 6. pp. 754-759.
@article{4781f7aa4ea94271988754a4d7de711f,
title = "Increased thrombogenicity in chronic renal failure in a rat model induced by 5/6 ablation/infarction",
abstract = "Purpose: Abnormalities in hemostasis and coagulation have been suggested in chronic renal failure (CRF). In this study, we compared processes of thrombus formation between rats with CRF and those with normal kidney function. Materials and Methods: CRF was induced by 5/6 ablation/infarction of the kidneys in Sprague-Dawley rats, and surviving rats after 4 weeks were used. Ferric chloride (FeCl3)-induced thrombosis in the carotid artery was induced to assess thrombus formation. Whole blood clot formation was evaluated using rotational thromboelastometry (ROTEM). Platelet aggregation was assessed with impedance platelet aggregometry. Results: FeCl3-induced thrombus formation was initiated faster in the CRF group than in the control group (13.2±1.1 sec vs. 17.8±1.0 sec, p=0.027). On histological examination, the maximal diameters of thrombi were larger in the CRF group than in the control group (394.2±201.1 μm vs. 114.0±145.1 μm, p=0.039). In extrinsic pathway ROTEM, the CRF group showed faster clot initiation (clotting time, 59.0±7.3 sec vs. 72.8±5.0 sec, p=0.032) and increased clot growth kinetics (α angle, 84.8±0.2° vs. 82.0±0.6°, p=0.008), compared to the control group. Maximal platelet aggregation rate was higher in the CRF group than in the control group (58.2±0.2{\%} vs. 44.6±1.2{\%}, p=0.006). Conclusion: Our study demonstrated that thrombogenicity is increased in rats with CRF. An activated extrinsic coagulation pathway may play an important role in increasing thrombogenicity in CRF.",
author = "Song, {Tae Jin} and Il Kwon and Honglim Piao and Lee, {Jee Eun} and Han, {Kyeo Rye} and Yoonkyung Chang and Oh, {Hyung Jung} and Choi, {Hyun Jung} and Lee, {Kyung Yul} and Kim, {Yong Jae} and Han, {Ki Hwan} and Heo, {Ji Hoe}",
year = "2018",
month = "8",
doi = "10.3349/ymj.2018.59.6.754",
language = "English",
volume = "59",
pages = "754--759",
journal = "Yonsei Medical Journal",
issn = "0513-5796",
publisher = "Yonsei University College of Medicine",
number = "6",

}

Song, TJ, Kwon, I, Piao, H, Lee, JE, Han, KR, Chang, Y, Oh, HJ, Choi, HJ, Lee, KY, Kim, YJ, Han, KH & Heo, JH 2018, 'Increased thrombogenicity in chronic renal failure in a rat model induced by 5/6 ablation/infarction', Yonsei medical journal, vol. 59, no. 6, pp. 754-759. https://doi.org/10.3349/ymj.2018.59.6.754

Increased thrombogenicity in chronic renal failure in a rat model induced by 5/6 ablation/infarction. / Song, Tae Jin; Kwon, Il; Piao, Honglim; Lee, Jee Eun; Han, Kyeo Rye; Chang, Yoonkyung; Oh, Hyung Jung; Choi, Hyun Jung; Lee, Kyung Yul; Kim, Yong Jae; Han, Ki Hwan; Heo, Ji Hoe.

In: Yonsei medical journal, Vol. 59, No. 6, 08.2018, p. 754-759.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Increased thrombogenicity in chronic renal failure in a rat model induced by 5/6 ablation/infarction

AU - Song, Tae Jin

AU - Kwon, Il

AU - Piao, Honglim

AU - Lee, Jee Eun

AU - Han, Kyeo Rye

AU - Chang, Yoonkyung

AU - Oh, Hyung Jung

AU - Choi, Hyun Jung

AU - Lee, Kyung Yul

AU - Kim, Yong Jae

AU - Han, Ki Hwan

AU - Heo, Ji Hoe

PY - 2018/8

Y1 - 2018/8

N2 - Purpose: Abnormalities in hemostasis and coagulation have been suggested in chronic renal failure (CRF). In this study, we compared processes of thrombus formation between rats with CRF and those with normal kidney function. Materials and Methods: CRF was induced by 5/6 ablation/infarction of the kidneys in Sprague-Dawley rats, and surviving rats after 4 weeks were used. Ferric chloride (FeCl3)-induced thrombosis in the carotid artery was induced to assess thrombus formation. Whole blood clot formation was evaluated using rotational thromboelastometry (ROTEM). Platelet aggregation was assessed with impedance platelet aggregometry. Results: FeCl3-induced thrombus formation was initiated faster in the CRF group than in the control group (13.2±1.1 sec vs. 17.8±1.0 sec, p=0.027). On histological examination, the maximal diameters of thrombi were larger in the CRF group than in the control group (394.2±201.1 μm vs. 114.0±145.1 μm, p=0.039). In extrinsic pathway ROTEM, the CRF group showed faster clot initiation (clotting time, 59.0±7.3 sec vs. 72.8±5.0 sec, p=0.032) and increased clot growth kinetics (α angle, 84.8±0.2° vs. 82.0±0.6°, p=0.008), compared to the control group. Maximal platelet aggregation rate was higher in the CRF group than in the control group (58.2±0.2% vs. 44.6±1.2%, p=0.006). Conclusion: Our study demonstrated that thrombogenicity is increased in rats with CRF. An activated extrinsic coagulation pathway may play an important role in increasing thrombogenicity in CRF.

AB - Purpose: Abnormalities in hemostasis and coagulation have been suggested in chronic renal failure (CRF). In this study, we compared processes of thrombus formation between rats with CRF and those with normal kidney function. Materials and Methods: CRF was induced by 5/6 ablation/infarction of the kidneys in Sprague-Dawley rats, and surviving rats after 4 weeks were used. Ferric chloride (FeCl3)-induced thrombosis in the carotid artery was induced to assess thrombus formation. Whole blood clot formation was evaluated using rotational thromboelastometry (ROTEM). Platelet aggregation was assessed with impedance platelet aggregometry. Results: FeCl3-induced thrombus formation was initiated faster in the CRF group than in the control group (13.2±1.1 sec vs. 17.8±1.0 sec, p=0.027). On histological examination, the maximal diameters of thrombi were larger in the CRF group than in the control group (394.2±201.1 μm vs. 114.0±145.1 μm, p=0.039). In extrinsic pathway ROTEM, the CRF group showed faster clot initiation (clotting time, 59.0±7.3 sec vs. 72.8±5.0 sec, p=0.032) and increased clot growth kinetics (α angle, 84.8±0.2° vs. 82.0±0.6°, p=0.008), compared to the control group. Maximal platelet aggregation rate was higher in the CRF group than in the control group (58.2±0.2% vs. 44.6±1.2%, p=0.006). Conclusion: Our study demonstrated that thrombogenicity is increased in rats with CRF. An activated extrinsic coagulation pathway may play an important role in increasing thrombogenicity in CRF.

UR - http://www.scopus.com/inward/record.url?scp=85050124089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050124089&partnerID=8YFLogxK

U2 - 10.3349/ymj.2018.59.6.754

DO - 10.3349/ymj.2018.59.6.754

M3 - Article

C2 - 29978612

AN - SCOPUS:85050124089

VL - 59

SP - 754

EP - 759

JO - Yonsei Medical Journal

JF - Yonsei Medical Journal

SN - 0513-5796

IS - 6

ER -