Increasing the resolution of light sheet microscopy in the presence of aberrations

T. Vettenburg, H. I.C. Dalgarno, T. Čižmár, F. J. Gunn-Moore, K. Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XX
DOIs
Publication statusPublished - 2013 May 28
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX - San Francisco, CA, United States
Duration: 2013 Feb 52013 Feb 7

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8589
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
CountryUnited States
CitySan Francisco, CA
Period13/2/513/2/7

Fingerprint

Aberrations
aberration
Microscopy
Microscopic examination
Tissue
microscopy
Light
Photobleaching
Three-Dimensional Imaging
Phase modulation
Pupil
Lighting
Diffraction
Imaging techniques
bleaching
pupils
phase modulation
micrometers
illumination
damage

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Vettenburg, T., Dalgarno, H. I. C., Čižmár, T., Gunn-Moore, F. J., & Dholakia, K. (2013). Increasing the resolution of light sheet microscopy in the presence of aberrations. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX [858912] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8589). https://doi.org/10.1117/12.2003828
Vettenburg, T. ; Dalgarno, H. I.C. ; Čižmár, T. ; Gunn-Moore, F. J. ; Dholakia, K. / Increasing the resolution of light sheet microscopy in the presence of aberrations. Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX. 2013. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{23c252acd6c64c27a24cf189f957519c,
title = "Increasing the resolution of light sheet microscopy in the presence of aberrations",
abstract = "Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.",
author = "T. Vettenburg and Dalgarno, {H. I.C.} and T. Čižm{\'a}r and Gunn-Moore, {F. J.} and K. Dholakia",
year = "2013",
month = "5",
day = "28",
doi = "10.1117/12.2003828",
language = "English",
isbn = "9780819493583",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Three-Dimensional and Multidimensional Microscopy",

}

Vettenburg, T, Dalgarno, HIC, Čižmár, T, Gunn-Moore, FJ & Dholakia, K 2013, Increasing the resolution of light sheet microscopy in the presence of aberrations. in Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX., 858912, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, San Francisco, CA, United States, 13/2/5. https://doi.org/10.1117/12.2003828

Increasing the resolution of light sheet microscopy in the presence of aberrations. / Vettenburg, T.; Dalgarno, H. I.C.; Čižmár, T.; Gunn-Moore, F. J.; Dholakia, K.

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX. 2013. 858912 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8589).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Increasing the resolution of light sheet microscopy in the presence of aberrations

AU - Vettenburg, T.

AU - Dalgarno, H. I.C.

AU - Čižmár, T.

AU - Gunn-Moore, F. J.

AU - Dholakia, K.

PY - 2013/5/28

Y1 - 2013/5/28

N2 - Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.

AB - Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.

UR - http://www.scopus.com/inward/record.url?scp=84878067482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878067482&partnerID=8YFLogxK

U2 - 10.1117/12.2003828

DO - 10.1117/12.2003828

M3 - Conference contribution

AN - SCOPUS:84878067482

SN - 9780819493583

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Three-Dimensional and Multidimensional Microscopy

ER -

Vettenburg T, Dalgarno HIC, Čižmár T, Gunn-Moore FJ, Dholakia K. Increasing the resolution of light sheet microscopy in the presence of aberrations. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX. 2013. 858912. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2003828