Increasing the resolution of light sheet microscopy in the presence of aberrations

T. Vettenburg, H. I.C. Dalgarno, T. Čižmár, F. J. Gunn-Moore, K. Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Single plane illumination microscopy (SPIM) allows rapid imaging of large, three-dimensional, samples of living tissue. The thin light sheet ensures high contrast whilst photo-bleaching and damage are kept to a minimum. However, many specimen of interest have a significant thickness. To date, high axial resolution in such specimen has only been achieved by compromising these key advantages and adding considerable technical complexity. Although the light sheet can propagate several hundreds of micrometers into the tissue, its width can be several orders of magnitude larger than it would be in a homogeneous sample. In this paper we explore the use of pupil-phase modulation to overcome such sample-induced aberrations and produce diffraction-limited deep inside turbid samples.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XX
DOIs
Publication statusPublished - 2013 May 28
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX - San Francisco, CA, United States
Duration: 2013 Feb 52013 Feb 7

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8589
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
CountryUnited States
CitySan Francisco, CA
Period13/2/513/2/7

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Vettenburg, T., Dalgarno, H. I. C., Čižmár, T., Gunn-Moore, F. J., & Dholakia, K. (2013). Increasing the resolution of light sheet microscopy in the presence of aberrations. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX [858912] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8589). https://doi.org/10.1117/12.2003828