Increasing the tissue thickness at implant sites using guided bone regeneration and an additional collagen matrix: Histologic observations in beagle dogs

Gi Young Seo, Daniel Stefan Thoma, Ui Won Jung, Jung Seok Lee

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Objectives: To histologically determine the alteration in horizontal mucosal thickness at sites that received guided bone regeneration (GBR) with additional use of collagen matrix and to assess whether bone formation is affected by adding collagen matrix at GBR sites at 8 weeks of healing. Materials and methods: Eight weeks after bilateral extraction of maxillary premolars, standardized defects were created on buccal side of edentulous ridges in four beagle dogs. One side was randomly allocated as control (biphasic calcium phosphate plus collagen membrane; GBR only), while contralateral side was allocated as test (biphasic calcium phosphate plus collagen membrane plus an additional layer of collagen matrix). Histologic observations, histomorphometric and micro-computed tomography analyses were performed after 8 weeks. Results: Membrane complex comprising residual collagen membrane and adjacent dense connective tissue was observed at both control and test sites. The thickness in the histologic analysis were 1.69 ± 0.23 mm (control) and 1.76 ± 0.07 mm (test) in histologic analysis and were 2.03 ± 0.26 mm (control) and 2.14 ± 0.24 mm (test) in radiographic analysis. The thickness of the membrane complex in soft-tissue layer were 723.0 ± 241.6 μm (control) and 984.6 ± 334.4 μm (test). The percentage of new bone formation were 22.30 ± 5.92% (control) and 25.50 ± 8.08% (test). All measured outcome did not show significant differences between control and test groups. Conclusion: The addition of collagen matrix on top of standard GBR procedure did not increase the soft tissue thickness and dense connective tissue formation at 8 weeks of healing. Bone regeneration was not affected by the addition of collagen matrix.

Original languageEnglish
Pages (from-to)741-749
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number3
Publication statusPublished - 2019 Apr

Bibliographical note

Funding Information:
Correspondence to: J.-S. Lee; e-mail: Contract grant sponsor: the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education; contract grant number: 2016R1C1B2015427 FIGURE 1. Clinical photographs of the surgical procedure at the test site. (a) Eight weeks after extraction, the flap was elevated. (b) Standardized defects (20 mm × 5 mm × 3 mm, width × height × depth) were created on the buccal side of each ridge. (c) BCP (comprising 30% hydroxyapatite and 70% β-tricalcium phosphate) was grafted and (d) a collagen membrane was applied over the defect. (e) An additional layer of collagen matrix was applied over the collagen membrane. (f ) Primary closure was then performed.

Publisher Copyright:
© 2018 Wiley Periodicals, Inc.

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering


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