Indolizino[3,2-c]quinolines as environment-sensitive fluorescent light-up probes for targeted live cell imaging

Soonbum Kwon, Dah In Kwon, Youngeun Jung, Ju Hee Kim, Yeongcheol Lee, Bumhee Lim, Ikyon Kim, Jeeyeon Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Live cell imaging using small organic fluorophores has emerged as a powerful non-invasive approach to visualizing a variety of cellular processes. We recently reported the design and synthesis of indolizino[3,2-c]quinolines (IQs), a fused heterocyclic system, as a potential candidate for novel fluorescent probes. Here, we explored the environment-sensitive features of the IQs in a cellular context using fluorescent microscopy to achieve no-wash live cell imaging with precise spatial control. Elucidating the cellular targets of IQs based on their chemical structures has enabled us to develop organelle-specific fluorophores that target DNA (3C2), RNA (3B1 and 3H1), and lysosomes (IQ-MP1 and IQ-MP2) in live cells. These compounds exhibit good cell membrane permeability, high photostability, and low cytotoxicity with good compatibility in aqueous systems, properties that are rarely encountered together. 3H1, with a large Stokes shift, exhibits counterstain compatibility when combined with DNA probes (Hoechst 33258) in live cells. The properties of the IQs described here suggest that these compounds may serve as important tools for investigating dynamic processes in live cells.

Original languageEnglish
Pages (from-to)340-352
Number of pages13
JournalSensors and Actuators, B: Chemical
Volume252
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Quinolines
Fluorophores
quinoline
DNA
Bisbenzimidazole
Imaging techniques
probes
DNA Probes
Cell membranes
Cytotoxicity
cells
RNA
Fluorescent Dyes
Microscopic examination
compatibility
deoxyribonucleic acid
lysosomes
organelles
permeability
microscopy

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Kwon, Soonbum ; Kwon, Dah In ; Jung, Youngeun ; Kim, Ju Hee ; Lee, Yeongcheol ; Lim, Bumhee ; Kim, Ikyon ; Lee, Jeeyeon. / Indolizino[3,2-c]quinolines as environment-sensitive fluorescent light-up probes for targeted live cell imaging. In: Sensors and Actuators, B: Chemical. 2017 ; Vol. 252. pp. 340-352.
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Indolizino[3,2-c]quinolines as environment-sensitive fluorescent light-up probes for targeted live cell imaging. / Kwon, Soonbum; Kwon, Dah In; Jung, Youngeun; Kim, Ju Hee; Lee, Yeongcheol; Lim, Bumhee; Kim, Ikyon; Lee, Jeeyeon.

In: Sensors and Actuators, B: Chemical, Vol. 252, 01.01.2017, p. 340-352.

Research output: Contribution to journalArticle

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