Induction of 1-aminocyclopropane-1-carboxylate oxidase mRNA by ethylene in mung bean roots

Possible involvement of Ca 2+ and phosphoinositides in ethylene signalling

T. Jung, J. H. Lee, M. H. Cho, Woo Taek Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Ethylene markedly induces an increase in the mRNA level of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, the final step of its biosynthetic pathway, in mung bean roots. To investigate the second messengers that possibly participate in ethylene signalling various pharmacological reagents known to affect the cytosolic calcium level and phosphoinositide (PI) metabolism were applied to mung bean roots, and then the induction pattern of ACC oxidase (VR-ACO1) by ethylene was monitored as a molecular paradigm for ethylene-dependent responses in this tissue. The ethylene-induced VR-ACO1 gene expression was effectively inhibited by ruthenium red, a putative inhibitor of intracellular Ca 2+ fluxes, and to a lesser extent by ethyleneglycotetraacetic acid, a calcium chelator and GdCl 3 , the plasma membrane calcium-channel inhibitor. However, LaCl 3 and verapamil, another type of plasma membrane calcium-channel blockers, did not prevent the ethylene action. The calmodulin antagonist W-7 significantly reduced the hormone-dependent expression of VR-ACO1. Treatment with neomycin or Li + , the antagonists of PI metabolism, almost completely blocked the ethylene-induced accumulation of VR-ACO1 transcript in a dose-dependent manner, whereas the addition of exogenous Ca 2+ along with antagonists effectively restored the ethylene-induced VR-ACO1 gene activation. The results showed that the ACC oxidase enzyme activities and protein levels were in parallel with the abundance of transcript in response to various antagonists and exogenous Ca 2+ . These results are discussed in light of suggestion that the PI-derived second messenger and cytosolic calcium are necessarily involved in the ethylene-induced ACC oxidase gene activation in mung bean root tissue.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalPlant, Cell and Environment
Volume23
Issue number2
DOIs
Publication statusPublished - 2000 Jan 1

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Phosphatidylinositols
mung beans
ethylene
Messenger RNA
antagonists
Oxidoreductases
gene activation
second messengers
Second Messenger Systems
Ion Channels
calcium
Transcriptional Activation
plasma membrane
Cell Membrane
Calcium
calcium channel blockers
1-aminocyclopropane-1-carboxylic acid oxidase
Vigna
Ruthenium Red
verapamil

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

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title = "Induction of 1-aminocyclopropane-1-carboxylate oxidase mRNA by ethylene in mung bean roots: Possible involvement of Ca 2+ and phosphoinositides in ethylene signalling",
abstract = "Ethylene markedly induces an increase in the mRNA level of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, the final step of its biosynthetic pathway, in mung bean roots. To investigate the second messengers that possibly participate in ethylene signalling various pharmacological reagents known to affect the cytosolic calcium level and phosphoinositide (PI) metabolism were applied to mung bean roots, and then the induction pattern of ACC oxidase (VR-ACO1) by ethylene was monitored as a molecular paradigm for ethylene-dependent responses in this tissue. The ethylene-induced VR-ACO1 gene expression was effectively inhibited by ruthenium red, a putative inhibitor of intracellular Ca 2+ fluxes, and to a lesser extent by ethyleneglycotetraacetic acid, a calcium chelator and GdCl 3 , the plasma membrane calcium-channel inhibitor. However, LaCl 3 and verapamil, another type of plasma membrane calcium-channel blockers, did not prevent the ethylene action. The calmodulin antagonist W-7 significantly reduced the hormone-dependent expression of VR-ACO1. Treatment with neomycin or Li + , the antagonists of PI metabolism, almost completely blocked the ethylene-induced accumulation of VR-ACO1 transcript in a dose-dependent manner, whereas the addition of exogenous Ca 2+ along with antagonists effectively restored the ethylene-induced VR-ACO1 gene activation. The results showed that the ACC oxidase enzyme activities and protein levels were in parallel with the abundance of transcript in response to various antagonists and exogenous Ca 2+ . These results are discussed in light of suggestion that the PI-derived second messenger and cytosolic calcium are necessarily involved in the ethylene-induced ACC oxidase gene activation in mung bean root tissue.",
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T1 - Induction of 1-aminocyclopropane-1-carboxylate oxidase mRNA by ethylene in mung bean roots

T2 - Possible involvement of Ca 2+ and phosphoinositides in ethylene signalling

AU - Jung, T.

AU - Lee, J. H.

AU - Cho, M. H.

AU - Kim, Woo Taek

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Ethylene markedly induces an increase in the mRNA level of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, the final step of its biosynthetic pathway, in mung bean roots. To investigate the second messengers that possibly participate in ethylene signalling various pharmacological reagents known to affect the cytosolic calcium level and phosphoinositide (PI) metabolism were applied to mung bean roots, and then the induction pattern of ACC oxidase (VR-ACO1) by ethylene was monitored as a molecular paradigm for ethylene-dependent responses in this tissue. The ethylene-induced VR-ACO1 gene expression was effectively inhibited by ruthenium red, a putative inhibitor of intracellular Ca 2+ fluxes, and to a lesser extent by ethyleneglycotetraacetic acid, a calcium chelator and GdCl 3 , the plasma membrane calcium-channel inhibitor. However, LaCl 3 and verapamil, another type of plasma membrane calcium-channel blockers, did not prevent the ethylene action. The calmodulin antagonist W-7 significantly reduced the hormone-dependent expression of VR-ACO1. Treatment with neomycin or Li + , the antagonists of PI metabolism, almost completely blocked the ethylene-induced accumulation of VR-ACO1 transcript in a dose-dependent manner, whereas the addition of exogenous Ca 2+ along with antagonists effectively restored the ethylene-induced VR-ACO1 gene activation. The results showed that the ACC oxidase enzyme activities and protein levels were in parallel with the abundance of transcript in response to various antagonists and exogenous Ca 2+ . These results are discussed in light of suggestion that the PI-derived second messenger and cytosolic calcium are necessarily involved in the ethylene-induced ACC oxidase gene activation in mung bean root tissue.

AB - Ethylene markedly induces an increase in the mRNA level of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, the final step of its biosynthetic pathway, in mung bean roots. To investigate the second messengers that possibly participate in ethylene signalling various pharmacological reagents known to affect the cytosolic calcium level and phosphoinositide (PI) metabolism were applied to mung bean roots, and then the induction pattern of ACC oxidase (VR-ACO1) by ethylene was monitored as a molecular paradigm for ethylene-dependent responses in this tissue. The ethylene-induced VR-ACO1 gene expression was effectively inhibited by ruthenium red, a putative inhibitor of intracellular Ca 2+ fluxes, and to a lesser extent by ethyleneglycotetraacetic acid, a calcium chelator and GdCl 3 , the plasma membrane calcium-channel inhibitor. However, LaCl 3 and verapamil, another type of plasma membrane calcium-channel blockers, did not prevent the ethylene action. The calmodulin antagonist W-7 significantly reduced the hormone-dependent expression of VR-ACO1. Treatment with neomycin or Li + , the antagonists of PI metabolism, almost completely blocked the ethylene-induced accumulation of VR-ACO1 transcript in a dose-dependent manner, whereas the addition of exogenous Ca 2+ along with antagonists effectively restored the ethylene-induced VR-ACO1 gene activation. The results showed that the ACC oxidase enzyme activities and protein levels were in parallel with the abundance of transcript in response to various antagonists and exogenous Ca 2+ . These results are discussed in light of suggestion that the PI-derived second messenger and cytosolic calcium are necessarily involved in the ethylene-induced ACC oxidase gene activation in mung bean root tissue.

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