Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells

Haemi Lee, Yun Jung Kim, Ha Won Kim, Dong Hee Lee, Mi Kyung Sung, Taesun Park

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Cordyceps militaris is a traditional herbal ingredient frequently used for tonic and medicinal purposes in eastern Asia. The hot water extract of its cultivated fruiting bodies demonstrated a potent cytotoxic effect against the proliferation of the human premyelocytic leukemia cell HL-60, with an IC 50 of 0.8 mg/ml for a 12-h treatment. It induced the characteristic apoptotic symptoms in the HL-60 cells, including DNA fragmentation and chromatin condensation, occurring within 12 - 16 h of treatment at a dose of 1 mg/ml. The activation of caspase-3 and the specific proteolytic cleavage of poly (ADP-ribose) polymerase were detected during the course of apoptosis induction. These results indicate that the hot water extract of Cordyceps militaris fruiting bodies inhibited cancer cell proliferation by inducing cell apoptosis through the activation of caspase-3, and that the Cordyceps militaris extract may therefore have therapeutic potential against human leukemia.

Original languageEnglish
Pages (from-to)670-674
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1

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Cordyceps
HL-60 Cells
Caspase 3
Leukemia
Apoptosis
Far East
Poly(ADP-ribose) Polymerases
Water
DNA Fragmentation
Chromatin
Cell Proliferation
Neoplasms
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Lee, Haemi ; Kim, Yun Jung ; Kim, Ha Won ; Lee, Dong Hee ; Sung, Mi Kyung ; Park, Taesun. / Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells. In: Biological and Pharmaceutical Bulletin. 2006 ; Vol. 29, No. 4. pp. 670-674.
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Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells. / Lee, Haemi; Kim, Yun Jung; Kim, Ha Won; Lee, Dong Hee; Sung, Mi Kyung; Park, Taesun.

In: Biological and Pharmaceutical Bulletin, Vol. 29, No. 4, 01.04.2006, p. 670-674.

Research output: Contribution to journalArticle

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