Induction of apoptosis by panduratin A isolated from Kaempferia pandurata in human colon cancer HT-29 cells

Jung Mi Yun, Hoonjeong Kwon, Hasan Mukhtar, Jae Kwan Hwang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We previously showed that panduratin A isolated from an extract of Kaempferia pandurata (Zingiberaceae) was a strong inhibitor of cyclooxygenase-2 (COX-2) in RAW264.7 cells, suggesting a potential use of panduratin A as an anti-inflammatory agent. In the present study, we have investigated the effects of panduratin A on cytoplasmic levels of COX-2, as well as proliferation and apoptosis in human colon cancer cells HT-29. Cell proliferation and induction of apoptosis was determined by the MTT assay, DNA fragmentation measurement, flow cytometric analysis, nuclear staining and Western blotting. The MTT assay indicated that panduratin A exhibited cytotoxicity with an IC50 value of 28 μM. The cytotoxic effects of panduratin A were found to be accompanied by the dose-dependent induction of apoptosis as assessed by DNA fragmentation and apoptotic bodies. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in cleavage of poly(ADP-ribose) polymerase (PARP) with a concomitant decrease in procaspase-3 protein. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in human colon cancer cells, suggesting its potential use as a cancer chemopreventive and therapeutic agent.

Original languageEnglish
Pages (from-to)501-507
Number of pages7
JournalPlanta Medica
Volume71
Issue number6
DOIs
Publication statusPublished - 2005 Jun 1

Fingerprint

Zingiberaceae
HT29 Cells
Colonic Neoplasms
Apoptosis
DNA Fragmentation
Assays
Cells
Poly(ADP-ribose) Polymerases
Cyclooxygenase 2 Inhibitors
DNA
Cell proliferation
Cell growth
Flow measurement
Cyclooxygenase 2
Cytotoxicity
panduratin A
Caspase 3
Inhibitory Concentration 50
Anti-Inflammatory Agents
Western Blotting

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

Yun, Jung Mi ; Kwon, Hoonjeong ; Mukhtar, Hasan ; Hwang, Jae Kwan. / Induction of apoptosis by panduratin A isolated from Kaempferia pandurata in human colon cancer HT-29 cells. In: Planta Medica. 2005 ; Vol. 71, No. 6. pp. 501-507.
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Induction of apoptosis by panduratin A isolated from Kaempferia pandurata in human colon cancer HT-29 cells. / Yun, Jung Mi; Kwon, Hoonjeong; Mukhtar, Hasan; Hwang, Jae Kwan.

In: Planta Medica, Vol. 71, No. 6, 01.06.2005, p. 501-507.

Research output: Contribution to journalArticle

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