Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells

Je Seon Song, Yong Duk Park, Jin Won Hyun, Jeong Hee Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fluorine compounds are widely used for the prevention of caries, and recently sodium fluorosilicate has been used in water fluorination. The cytotoxic effects of sodium fluorosilicate in several osteosarcoma and oral cancer cells were evaluated in this study by measurement of inhibition of cell proliferation. Human osteogenic sarcoma (HOS) cells were the most sensitive to sodium fluorosilicate treatment. Induction of apoptosis, such as nucleosomal DNA fragmentation and the appearance of apoptotic bodies, were observed in HOS cells by agarose gel electrophoresis and by flow cytometric analysis, respectively. The molecular mechanism of apoptosis induction in HOS was investigated by Western blot analysis. The level of Bcl-2 was decreased and consequent release of cytochrome c was increased. Caspase-3 was activated and the cleavage of poly (ADP-ribosyl) polymerase was increased. In conclusion, sodium fluorosilicate induces apoptosis in HOS cells through decrease in Bcl-2, the release of cytochrome c to the cytosol and activation of caspase-3.

Original languageEnglish
Pages (from-to)391-395
Number of pages5
JournalAnticancer research
Volume25
Issue number1 A
Publication statusPublished - 2005 Jan 1

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Osteosarcoma
Apoptosis
Cytochromes c
Caspase 3
Fluorine Compounds
Agar Gel Electrophoresis
Halogenation
Mouth Neoplasms
DNA Fragmentation
Cytosol
Adenosine Diphosphate
Western Blotting
Cell Proliferation
hexafluorosilicate
Water

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Song, Je Seon ; Park, Yong Duk ; Hyun, Jin Won ; Kim, Jeong Hee. / Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells. In: Anticancer research. 2005 ; Vol. 25, No. 1 A. pp. 391-395.
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Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells. / Song, Je Seon; Park, Yong Duk; Hyun, Jin Won; Kim, Jeong Hee.

In: Anticancer research, Vol. 25, No. 1 A, 01.01.2005, p. 391-395.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells

AU - Song, Je Seon

AU - Park, Yong Duk

AU - Hyun, Jin Won

AU - Kim, Jeong Hee

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N2 - Fluorine compounds are widely used for the prevention of caries, and recently sodium fluorosilicate has been used in water fluorination. The cytotoxic effects of sodium fluorosilicate in several osteosarcoma and oral cancer cells were evaluated in this study by measurement of inhibition of cell proliferation. Human osteogenic sarcoma (HOS) cells were the most sensitive to sodium fluorosilicate treatment. Induction of apoptosis, such as nucleosomal DNA fragmentation and the appearance of apoptotic bodies, were observed in HOS cells by agarose gel electrophoresis and by flow cytometric analysis, respectively. The molecular mechanism of apoptosis induction in HOS was investigated by Western blot analysis. The level of Bcl-2 was decreased and consequent release of cytochrome c was increased. Caspase-3 was activated and the cleavage of poly (ADP-ribosyl) polymerase was increased. In conclusion, sodium fluorosilicate induces apoptosis in HOS cells through decrease in Bcl-2, the release of cytochrome c to the cytosol and activation of caspase-3.

AB - Fluorine compounds are widely used for the prevention of caries, and recently sodium fluorosilicate has been used in water fluorination. The cytotoxic effects of sodium fluorosilicate in several osteosarcoma and oral cancer cells were evaluated in this study by measurement of inhibition of cell proliferation. Human osteogenic sarcoma (HOS) cells were the most sensitive to sodium fluorosilicate treatment. Induction of apoptosis, such as nucleosomal DNA fragmentation and the appearance of apoptotic bodies, were observed in HOS cells by agarose gel electrophoresis and by flow cytometric analysis, respectively. The molecular mechanism of apoptosis induction in HOS was investigated by Western blot analysis. The level of Bcl-2 was decreased and consequent release of cytochrome c was increased. Caspase-3 was activated and the cleavage of poly (ADP-ribosyl) polymerase was increased. In conclusion, sodium fluorosilicate induces apoptosis in HOS cells through decrease in Bcl-2, the release of cytochrome c to the cytosol and activation of caspase-3.

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