Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models

J. C. Park, S. S. So, I. H. Jung, J. H. Yun, Seongho Choi, K. S. Cho, ChangSung Kim

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Abstract

Objective: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. Material and Methods: Critical-size (Φ8mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results: ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8wk (10.8% and 25.4%, respectively) than the control at 2 and 8wk (5.3% and 14.0%, respectively) in calvarial defects (p<0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8wk (7.5%) than at 2wk (4.5%) (p<0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8wk. The bMBCP carrier showed no evidence of bioresorption. Conclusion: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.

Original languageEnglish
Pages (from-to)682-690
Number of pages9
JournalJournal of Periodontal Research
Volume46
Issue number6
DOIs
Publication statusPublished - 2011 Dec 1

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Osteogenesis
Phosphate Transport Proteins
Escherichia coli
recombinant human bone morphogenetic protein-2
hydroxyapatite-beta tricalcium phosphate
Sprague Dawley Rats
Bone Marrow

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

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title = "Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models",
abstract = "Objective: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. Material and Methods: Critical-size (Φ8mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results: ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8wk (10.8{\%} and 25.4{\%}, respectively) than the control at 2 and 8wk (5.3{\%} and 14.0{\%}, respectively) in calvarial defects (p<0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8wk (7.5{\%}) than at 2wk (4.5{\%}) (p<0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8wk. The bMBCP carrier showed no evidence of bioresorption. Conclusion: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.",
author = "Park, {J. C.} and So, {S. S.} and Jung, {I. H.} and Yun, {J. H.} and Seongho Choi and Cho, {K. S.} and ChangSung Kim",
year = "2011",
month = "12",
day = "1",
doi = "10.1111/j.1600-0765.2011.01390.x",
language = "English",
volume = "46",
pages = "682--690",
journal = "Journal of Periodontal Research",
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TY - JOUR

T1 - Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models

AU - Park, J. C.

AU - So, S. S.

AU - Jung, I. H.

AU - Yun, J. H.

AU - Choi, Seongho

AU - Cho, K. S.

AU - Kim, ChangSung

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Objective: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. Material and Methods: Critical-size (Φ8mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results: ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8wk (10.8% and 25.4%, respectively) than the control at 2 and 8wk (5.3% and 14.0%, respectively) in calvarial defects (p<0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8wk (7.5%) than at 2wk (4.5%) (p<0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8wk. The bMBCP carrier showed no evidence of bioresorption. Conclusion: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.

AB - Objective: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. Material and Methods: Critical-size (Φ8mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results: ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8wk (10.8% and 25.4%, respectively) than the control at 2 and 8wk (5.3% and 14.0%, respectively) in calvarial defects (p<0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8wk (7.5%) than at 2wk (4.5%) (p<0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8wk. The bMBCP carrier showed no evidence of bioresorption. Conclusion: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.

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U2 - 10.1111/j.1600-0765.2011.01390.x

DO - 10.1111/j.1600-0765.2011.01390.x

M3 - Article

VL - 46

SP - 682

EP - 690

JO - Journal of Periodontal Research

JF - Journal of Periodontal Research

SN - 0022-3484

IS - 6

ER -