Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models

J. C. Park, S. S. So, I. H. Jung, J. H. Yun, S. H. Choi, K. S. Cho, C. S. Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)


Objective: The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. Material and Methods: Critical-size (Φ8mm) calvarial defects and subcutaneous pockets in 32 Sprague-Dawley rats received implants of rhBMP-2 (2.5μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results: ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8wk (10.8% and 25.4%, respectively) than the control at 2 and 8wk (5.3% and 14.0%, respectively) in calvarial defects (p<0.01). Bone formation was only observed for the ErhBMP-2/bMBCP ectopic sites and was significantly greater at 8wk (7.5%) than at 2wk (4.5%) (p<0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8wk. The bMBCP carrier showed no evidence of bioresorption. Conclusion: ErhBMP-2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.

Original languageEnglish
Pages (from-to)682-690
Number of pages9
JournalJournal of Periodontal Research
Issue number6
Publication statusPublished - 2011 Dec 1


All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this