Induction of cell apoptosis in non-small cell lung cancer cells by cyclin A1 small interfering RNA

Nam Hoon Cho, Yoon Pyo Choi, Dong Suk Moon, Haeryoung Kim, Suki Kang, Owen Ding, Sun Young Rha, Yeon Ju Yang, Sang Ho Cho

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cyclin A1 and cyclin B1 are overexpressed in various tumors but are present at low levels in normal tissues. Cyclin A1 is restricted to germ cells undergoing meiosis. In order to explore the possibility of using cyclin A1 and cyclin B1 as anticancer targets, we knocked them down in two lung cancer cell lines, H157 and H596, using siRNA. As with cyclin A1 siRNA in lung cancer cell lines, cyclin B1, Cdc2 and CDK2 were all significantly downregulated. The S phase fraction increased significantly, and they eventually underwent apoptosis by way of downregulated intrinsic apoptotic pathways and modulators with upregulated extrinsic apoptotic pathways. Our study suggests that cyclin A1 might be a promising anticancer target specific to lung cancer.

Original languageEnglish
Pages (from-to)1082-1092
Number of pages11
JournalCancer Science
Volume97
Issue number10
DOIs
Publication statusPublished - 2006 Oct 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this