Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer

Se Hoon Kim, Jung Eun Lee, Seung Ho Yang, Sang Won Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The association between rapamycin and astrocytes in a tumor-bearing mouse model with brain metastases of non--small cell lung cancer (NSCLC) was investigated. For in vitro experiments, NCI-H358, a human lung adenocarcinoma cell line, was co-cultured with immortalized astrocytes, and treated with rapamycin, an mTOR inhibitor. We evaluated the expression of interleukin-1 (IL-1), interleukin-3 (IL-3), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), insulin-like growth factor-1 (IGF-1), platelet-derived growth factor (PDGF), chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1) in tumor cells in vivo. Rapamycin is cytotoxic in vitro; however, co-culturing tumor cells and astrocytes induced tumor cell survival. IL-1, IL-3, IL-6, TNF-α, TGF-β, PDGF, MCP-1 and MIP-1 expression were higher in rapamycin-treated mice compared to the control group, however, IGF-1 expression was lower. Notably, treatment with rapamycin before inoculating tumor cells affected cytokine expression in the tumor microenvironment. We suggest that growth factors and cytokines in the tumor microenvironment play a role in the survival of cancer cells in brain metastases.

Original languageEnglish
Pages (from-to)953-958
Number of pages6
JournalOncology Letters
Volume5
Issue number3
DOIs
Publication statusPublished - 2013 Mar 1

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Sirolimus
Lung Neoplasms
Intercellular Signaling Peptides and Proteins
Cytokines
Neoplasm Metastasis
Brain
Astrocytes
Macrophage Inflammatory Proteins
Neoplasms
Tumor Microenvironment
Interleukin-3
Platelet-Derived Growth Factor
Transforming Growth Factors
Somatomedins
Interleukin-1
Interleukin-6
Cell Survival
Tumor Necrosis Factor-alpha
Chemotactic Factors
Non-Small Cell Lung Carcinoma

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kim, Se Hoon ; Lee, Jung Eun ; Yang, Seung Ho ; Lee, Sang Won. / Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer. In: Oncology Letters. 2013 ; Vol. 5, No. 3. pp. 953-958.
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abstract = "The association between rapamycin and astrocytes in a tumor-bearing mouse model with brain metastases of non--small cell lung cancer (NSCLC) was investigated. For in vitro experiments, NCI-H358, a human lung adenocarcinoma cell line, was co-cultured with immortalized astrocytes, and treated with rapamycin, an mTOR inhibitor. We evaluated the expression of interleukin-1 (IL-1), interleukin-3 (IL-3), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), insulin-like growth factor-1 (IGF-1), platelet-derived growth factor (PDGF), chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1) in tumor cells in vivo. Rapamycin is cytotoxic in vitro; however, co-culturing tumor cells and astrocytes induced tumor cell survival. IL-1, IL-3, IL-6, TNF-α, TGF-β, PDGF, MCP-1 and MIP-1 expression were higher in rapamycin-treated mice compared to the control group, however, IGF-1 expression was lower. Notably, treatment with rapamycin before inoculating tumor cells affected cytokine expression in the tumor microenvironment. We suggest that growth factors and cytokines in the tumor microenvironment play a role in the survival of cancer cells in brain metastases.",
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Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer. / Kim, Se Hoon; Lee, Jung Eun; Yang, Seung Ho; Lee, Sang Won.

In: Oncology Letters, Vol. 5, No. 3, 01.03.2013, p. 953-958.

Research output: Contribution to journalArticle

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