Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer

SeHoon Kim, Jung Eun Lee, Seung Ho Yang, Sang Won Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The association between rapamycin and astrocytes in a tumor-bearing mouse model with brain metastases of non--small cell lung cancer (NSCLC) was investigated. For in vitro experiments, NCI-H358, a human lung adenocarcinoma cell line, was co-cultured with immortalized astrocytes, and treated with rapamycin, an mTOR inhibitor. We evaluated the expression of interleukin-1 (IL-1), interleukin-3 (IL-3), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), insulin-like growth factor-1 (IGF-1), platelet-derived growth factor (PDGF), chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1) in tumor cells in vivo. Rapamycin is cytotoxic in vitro; however, co-culturing tumor cells and astrocytes induced tumor cell survival. IL-1, IL-3, IL-6, TNF-α, TGF-β, PDGF, MCP-1 and MIP-1 expression were higher in rapamycin-treated mice compared to the control group, however, IGF-1 expression was lower. Notably, treatment with rapamycin before inoculating tumor cells affected cytokine expression in the tumor microenvironment. We suggest that growth factors and cytokines in the tumor microenvironment play a role in the survival of cancer cells in brain metastases.

Original languageEnglish
Pages (from-to)953-958
Number of pages6
JournalOncology Letters
Volume5
Issue number3
DOIs
Publication statusPublished - 2013 Mar 1

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Sirolimus
Lung Neoplasms
Intercellular Signaling Peptides and Proteins
Cytokines
Neoplasm Metastasis
Brain
Astrocytes
Macrophage Inflammatory Proteins
Neoplasms
Tumor Microenvironment
Interleukin-3
Platelet-Derived Growth Factor
Transforming Growth Factors
Somatomedins
Interleukin-1
Interleukin-6
Cell Survival
Tumor Necrosis Factor-alpha
Chemotactic Factors
Non-Small Cell Lung Carcinoma

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kim, SeHoon ; Lee, Jung Eun ; Yang, Seung Ho ; Lee, Sang Won. / Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer. In: Oncology Letters. 2013 ; Vol. 5, No. 3. pp. 953-958.
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abstract = "The association between rapamycin and astrocytes in a tumor-bearing mouse model with brain metastases of non--small cell lung cancer (NSCLC) was investigated. For in vitro experiments, NCI-H358, a human lung adenocarcinoma cell line, was co-cultured with immortalized astrocytes, and treated with rapamycin, an mTOR inhibitor. We evaluated the expression of interleukin-1 (IL-1), interleukin-3 (IL-3), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), insulin-like growth factor-1 (IGF-1), platelet-derived growth factor (PDGF), chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1) in tumor cells in vivo. Rapamycin is cytotoxic in vitro; however, co-culturing tumor cells and astrocytes induced tumor cell survival. IL-1, IL-3, IL-6, TNF-α, TGF-β, PDGF, MCP-1 and MIP-1 expression were higher in rapamycin-treated mice compared to the control group, however, IGF-1 expression was lower. Notably, treatment with rapamycin before inoculating tumor cells affected cytokine expression in the tumor microenvironment. We suggest that growth factors and cytokines in the tumor microenvironment play a role in the survival of cancer cells in brain metastases.",
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Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer. / Kim, SeHoon; Lee, Jung Eun; Yang, Seung Ho; Lee, Sang Won.

In: Oncology Letters, Vol. 5, No. 3, 01.03.2013, p. 953-958.

Research output: Contribution to journalArticle

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