Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Sang Choel Lee, Seung Hyeok Han, Jin Ji Li, Sun Ha Lee, Dong Sub Jung, Seung Jae Kwak, Seung Hye Kim, Dong Ki Kim, Tae Hyun Yoo, Jin Hyun Kim, Se Ho Chang, Dae Suk Han, Shin Wook Kang

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Abstract

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Original languageEnglish
Pages (from-to)838-848
Number of pages11
JournalKidney International
Volume76
Issue number8
DOIs
Publication statusPublished - 2009 Oct

All Science Journal Classification (ASJC) codes

  • Nephrology

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    Lee, S. C., Han, S. H., Li, J. J., Lee, S. H., Jung, D. S., Kwak, S. J., Kim, S. H., Kim, D. K., Yoo, T. H., Kim, J. H., Chang, S. H., Han, D. S., & Kang, S. W. (2009). Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions. Kidney International, 76(8), 838-848. https://doi.org/10.1038/ki.2009.286