Abstract
Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 838-848 |
| Number of pages | 11 |
| Journal | Kidney International |
| Volume | 76 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2009 Oct 1 |
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All Science Journal Classification (ASJC) codes
- Nephrology
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Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions. / Lee, Sang Choel; Han, SeungHyeok; Li, Jin Ji; Lee, Sun Ha; Jung, Dong Sub; Kwak, Seung Jae; Kim, Seung Hye; Kim, Dong Ki; Yoo, TaeHyun; Kim, Jin Hyun; Chang, Se Ho; Han, Dae Suk; Kang, Shin-Wook.
In: Kidney International, Vol. 76, No. 8, 01.10.2009, p. 838-848.Research output: Contribution to journal › Article
TY - JOUR
T1 - Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions
AU - Lee, Sang Choel
AU - Han, SeungHyeok
AU - Li, Jin Ji
AU - Lee, Sun Ha
AU - Jung, Dong Sub
AU - Kwak, Seung Jae
AU - Kim, Seung Hye
AU - Kim, Dong Ki
AU - Yoo, TaeHyun
AU - Kim, Jin Hyun
AU - Chang, Se Ho
AU - Han, Dae Suk
AU - Kang, Shin-Wook
PY - 2009/10/1
Y1 - 2009/10/1
N2 - Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.
AB - Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.
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U2 - 10.1038/ki.2009.286
DO - 10.1038/ki.2009.286
M3 - Article
VL - 76
SP - 838
EP - 848
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 8
ER -