Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Sang Choel Lee, SeungHyeok Han, Jin Ji Li, Sun Ha Lee, Dong Sub Jung, Seung Jae Kwak, Seung Hye Kim, Dong Ki Kim, TaeHyun Yoo, Jin Hyun Kim, Se Ho Chang, Dae Suk Han, Shin-Wook Kang

Research output: Contribution to journalArticle

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Abstract

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Original languageEnglish
Pages (from-to)838-848
Number of pages11
JournalKidney International
Volume76
Issue number8
DOIs
Publication statusPublished - 2009 Oct 1

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Podocytes
Heme Oxygenase-1
Apoptosis
Oxidants
Glucose
Streptozocin
Caspase 3
Small Interfering RNA
Cell Count
Wounds and Injuries
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Lee, Sang Choel ; Han, SeungHyeok ; Li, Jin Ji ; Lee, Sun Ha ; Jung, Dong Sub ; Kwak, Seung Jae ; Kim, Seung Hye ; Kim, Dong Ki ; Yoo, TaeHyun ; Kim, Jin Hyun ; Chang, Se Ho ; Han, Dae Suk ; Kang, Shin-Wook. / Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions. In: Kidney International. 2009 ; Vol. 76, No. 8. pp. 838-848.
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abstract = "Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.",
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Lee, SC, Han, S, Li, JJ, Lee, SH, Jung, DS, Kwak, SJ, Kim, SH, Kim, DK, Yoo, T, Kim, JH, Chang, SH, Han, DS & Kang, S-W 2009, 'Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions', Kidney International, vol. 76, no. 8, pp. 838-848. https://doi.org/10.1038/ki.2009.286

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions. / Lee, Sang Choel; Han, SeungHyeok; Li, Jin Ji; Lee, Sun Ha; Jung, Dong Sub; Kwak, Seung Jae; Kim, Seung Hye; Kim, Dong Ki; Yoo, TaeHyun; Kim, Jin Hyun; Chang, Se Ho; Han, Dae Suk; Kang, Shin-Wook.

In: Kidney International, Vol. 76, No. 8, 01.10.2009, p. 838-848.

Research output: Contribution to journalArticle

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AU - Han, SeungHyeok

AU - Li, Jin Ji

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AU - Kwak, Seung Jae

AU - Kim, Seung Hye

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AU - Yoo, TaeHyun

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AU - Chang, Se Ho

AU - Han, Dae Suk

AU - Kang, Shin-Wook

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