Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Sang Choel Lee; Seung Hyeok Han; Jin Ji Li; Sun Ha Lee; Dong Sub Jung; Seung Jae Kwak; Seung Hye Kim; Dong Ki Kim; Tae Hyun Yoo; Jin Hyun Kim; Se Ho Chang; Dae Suk Han; Shin Wook Kang

doi:10.1038/ki.2009.286

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Sang Choel Lee, Seung Hyeok Han, Jin Ji Li, Sun Ha Lee, Dong Sub Jung, Seung Jae Kwak, Seung Hye Kim, Dong Ki Kim, Tae Hyun Yoo, Jin Hyun Kim, Se Ho Chang, Dae Suk Han, Shin Wook Kang

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Original language	English
Pages (from-to)	838-848
Number of pages	11
Journal	Kidney International
Volume	76
Issue number	8
DOIs	https://doi.org/10.1038/ki.2009.286
Publication status	Published - 2009 Oct

Bibliographical note

Funding Information:
This work was supported in part by the BK21 (Brain Korea 21) Project for Medical Sciences, Yonsei University, and the Korean Science and Engineering Foundation grant funded by the Korean government (R01-2007-000-20263-0 and R13-2002-054-04001-0).

All Science Journal Classification (ASJC) codes

Nephrology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/ki.2009.286

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title = "Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions",

abstract = "Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.",

author = "Lee, {Sang Choel} and Han, {Seung Hyeok} and Li, {Jin Ji} and Lee, {Sun Ha} and Jung, {Dong Sub} and Kwak, {Seung Jae} and Kim, {Seung Hye} and Kim, {Dong Ki} and Yoo, {Tae Hyun} and Kim, {Jin Hyun} and Chang, {Se Ho} and Han, {Dae Suk} and Kang, {Shin Wook}",

note = "Funding Information: This work was supported in part by the BK21 (Brain Korea 21) Project for Medical Sciences, Yonsei University, and the Korean Science and Engineering Foundation grant funded by the Korean government (R01-2007-000-20263-0 and R13-2002-054-04001-0).",

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AU - Kwak, Seung Jae

AU - Kim, Seung Hye

AU - Kim, Dong Ki

AU - Yoo, Tae Hyun

AU - Kim, Jin Hyun

AU - Chang, Se Ho

AU - Han, Dae Suk

AU - Kang, Shin Wook

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Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Abstract

Bibliographical note

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