Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells

Ga Yeon Son, Jeong Hee Hong, Inik Chang, DongMin Shin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: The oral cavity is often exposed to not only diverse external pathogens but also dramatic temperature changes. In this study, we investigated the effect of thermal stress on PDL cells with a focus on the inflammatory responses and bone homeostasis. Design: The PDL cells were isolated from healthy premolar extracted for orthodontic reasons, and examined using intracellular calcium concentration ([Ca 2+ ] i ) measurement and reverse transcription-polymerase chain reaction for pro-inflammatory cytokines and bone remodelling mediators. Results: We detected the expression of thermosensitive transient receptor potential (TRP) channels, such as TRPV1, TRPV2, TRPV3, TRPM8, and TRPA1. Functional activation of the channels by thermal stress and their specific agonists increased [Ca 2+ ] i and interleukin (IL)-6 and IL-8 mRNA expression. A selective Ca 2+ chelator, BAPTA-AM, prevented TRP channel agonists-mediated IL-6 and IL-8 induction. Unlike pro-inflammatory cytokines, the expression of bone remodelling mediators, including receptor activator of nuclear factor-kappa B ligand and osteoprotegerin, was not altered by treatment with TRP channel agonists. Conclusions: The activation of thermosensitive TRP channels induced IL-6 and IL-8 expression by increasing [Ca 2+ ] i in human PDL cells. Therefore, thermal stress may play a critical role in the inflammatory responses of PDL cells.

Original languageEnglish
Pages (from-to)526-532
Number of pages7
JournalArchives of Oral Biology
Volume60
Issue number4
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Transient Receptor Potential Channels
Interleukin-8
Interleukin-6
Hot Temperature
Bone Remodeling
Cytokines
RANK Ligand
Osteoprotegerin
Bicuspid
Chelating Agents
Orthodontics
Reverse Transcription
Mouth
Homeostasis
Calcium
Bone and Bones
Polymerase Chain Reaction
Messenger RNA
Temperature

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Son, Ga Yeon ; Hong, Jeong Hee ; Chang, Inik ; Shin, DongMin. / Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells. In: Archives of Oral Biology. 2015 ; Vol. 60, No. 4. pp. 526-532.
@article{afa95309a34c464190cae367026e1586,
title = "Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells",
abstract = "Objective: The oral cavity is often exposed to not only diverse external pathogens but also dramatic temperature changes. In this study, we investigated the effect of thermal stress on PDL cells with a focus on the inflammatory responses and bone homeostasis. Design: The PDL cells were isolated from healthy premolar extracted for orthodontic reasons, and examined using intracellular calcium concentration ([Ca 2+ ] i ) measurement and reverse transcription-polymerase chain reaction for pro-inflammatory cytokines and bone remodelling mediators. Results: We detected the expression of thermosensitive transient receptor potential (TRP) channels, such as TRPV1, TRPV2, TRPV3, TRPM8, and TRPA1. Functional activation of the channels by thermal stress and their specific agonists increased [Ca 2+ ] i and interleukin (IL)-6 and IL-8 mRNA expression. A selective Ca 2+ chelator, BAPTA-AM, prevented TRP channel agonists-mediated IL-6 and IL-8 induction. Unlike pro-inflammatory cytokines, the expression of bone remodelling mediators, including receptor activator of nuclear factor-kappa B ligand and osteoprotegerin, was not altered by treatment with TRP channel agonists. Conclusions: The activation of thermosensitive TRP channels induced IL-6 and IL-8 expression by increasing [Ca 2+ ] i in human PDL cells. Therefore, thermal stress may play a critical role in the inflammatory responses of PDL cells.",
author = "Son, {Ga Yeon} and Hong, {Jeong Hee} and Inik Chang and DongMin Shin",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.archoralbio.2014.12.014",
language = "English",
volume = "60",
pages = "526--532",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "4",

}

Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells. / Son, Ga Yeon; Hong, Jeong Hee; Chang, Inik; Shin, DongMin.

In: Archives of Oral Biology, Vol. 60, No. 4, 01.01.2015, p. 526-532.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells

AU - Son, Ga Yeon

AU - Hong, Jeong Hee

AU - Chang, Inik

AU - Shin, DongMin

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Objective: The oral cavity is often exposed to not only diverse external pathogens but also dramatic temperature changes. In this study, we investigated the effect of thermal stress on PDL cells with a focus on the inflammatory responses and bone homeostasis. Design: The PDL cells were isolated from healthy premolar extracted for orthodontic reasons, and examined using intracellular calcium concentration ([Ca 2+ ] i ) measurement and reverse transcription-polymerase chain reaction for pro-inflammatory cytokines and bone remodelling mediators. Results: We detected the expression of thermosensitive transient receptor potential (TRP) channels, such as TRPV1, TRPV2, TRPV3, TRPM8, and TRPA1. Functional activation of the channels by thermal stress and their specific agonists increased [Ca 2+ ] i and interleukin (IL)-6 and IL-8 mRNA expression. A selective Ca 2+ chelator, BAPTA-AM, prevented TRP channel agonists-mediated IL-6 and IL-8 induction. Unlike pro-inflammatory cytokines, the expression of bone remodelling mediators, including receptor activator of nuclear factor-kappa B ligand and osteoprotegerin, was not altered by treatment with TRP channel agonists. Conclusions: The activation of thermosensitive TRP channels induced IL-6 and IL-8 expression by increasing [Ca 2+ ] i in human PDL cells. Therefore, thermal stress may play a critical role in the inflammatory responses of PDL cells.

AB - Objective: The oral cavity is often exposed to not only diverse external pathogens but also dramatic temperature changes. In this study, we investigated the effect of thermal stress on PDL cells with a focus on the inflammatory responses and bone homeostasis. Design: The PDL cells were isolated from healthy premolar extracted for orthodontic reasons, and examined using intracellular calcium concentration ([Ca 2+ ] i ) measurement and reverse transcription-polymerase chain reaction for pro-inflammatory cytokines and bone remodelling mediators. Results: We detected the expression of thermosensitive transient receptor potential (TRP) channels, such as TRPV1, TRPV2, TRPV3, TRPM8, and TRPA1. Functional activation of the channels by thermal stress and their specific agonists increased [Ca 2+ ] i and interleukin (IL)-6 and IL-8 mRNA expression. A selective Ca 2+ chelator, BAPTA-AM, prevented TRP channel agonists-mediated IL-6 and IL-8 induction. Unlike pro-inflammatory cytokines, the expression of bone remodelling mediators, including receptor activator of nuclear factor-kappa B ligand and osteoprotegerin, was not altered by treatment with TRP channel agonists. Conclusions: The activation of thermosensitive TRP channels induced IL-6 and IL-8 expression by increasing [Ca 2+ ] i in human PDL cells. Therefore, thermal stress may play a critical role in the inflammatory responses of PDL cells.

UR - http://www.scopus.com/inward/record.url?scp=84920973038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920973038&partnerID=8YFLogxK

U2 - 10.1016/j.archoralbio.2014.12.014

DO - 10.1016/j.archoralbio.2014.12.014

M3 - Article

VL - 60

SP - 526

EP - 532

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 4

ER -