Inflammation, impaired motility, and permeability in a Guinea Pig model of postoperative ileus

Yoo Jin Lee, Zahid Hussain, Cheal Wung Huh, Young Ju Lee, Hyojin Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background/Aims Postoperative ileus (POI) is characterized by impaired propulsive function of the gastrointestinal tract after surgery. Although inflammation is considered to be an important pathogenesis of POI, significant data are lacking. We aim to correlate the recovery time of postoperative dysmotility with that of inflammation and mucosal permeability. Methods An experimental POI model of guinea pig was used. Contractile activity of the circular muscle of the stomach, jejunum, ileum, and proximal colon was measured through a tissue bath study. Inflammatory cells were counted, and the expression of calprotectin and tryptase were analyzed. The expression of protease-activated receptor 2 (PAR-2), claudin-1, and claudin-2 were analyzed with immunofluorescence. Results The small bowel and colon showed decreased contractile amplitude in the POI groups compared to control. In contrast to the colon, the contractile amplitude of the small bowel significantly recovered in the POI group at 6 hours after the operation compared to the control group. Inflammation was highly significant in the POI groups compared to the control and sham groups, especially in the colon. Immunofluorescence showed increased PAR-2 expression in the POI groups compared to sham. The decreased claudin-1 expression and increased claudin-2 expression may suggest increased mucosal permeability of the small bowel and colon in the POI groups. Conclusions Increased inflammation and mucosal permeability may play an important role in the differential recovery stages in POI. These data may provide further insights into the pathophysiology and potential new therapeutic prospects of POI.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalJournal of Neurogastroenterology and Motility
Volume24
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

Bibliographical note

Funding Information:
Financial support: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant No. R1A1A2).

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Gastroenterology

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