Abstract
Purpose: Small intestinal function returns first after surgery, and then the function of the stomach returns to normal after postoperative ileus (POI). The aim of this study was to investigate inflammatory responses in the muscle coat of stomach and small intestine in guinea pig POI model. Materials and Methods: The distance of charcoal migration from pylorus to the distal intestine was measured. Hematoxylin and eosin (H&E) and immunohistochemical stain for calprotectin were done from the histologic sections of stomach, jejunum and ileum obtained at 3 and 6 hour after operation. Data were compared between sham operation and POI groups. Results: The distance of charcoal migration was significantly reduced in the 3 and 6 hour POI groups compared with sham operated groups (p<0.05). On H&E staining, the degree of inflammation was significantly higher in the stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). Calprotectin positive cells were significantly increased in the muscle coat of stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). There was strong association between the degree of inflammation and calprotectin positive cells in stomach. Conclusion: Postoperative ileus induced by cecal manipulation significantly increased the degree of inflammation and calprotectin positive cells in the muscle coat of stomach as a remote organ. The relevance of degree of inflammation and the recovery time of ileus should be pursued in the future research.
| Original language | English |
|---|---|
| Pages (from-to) | 1336-1341 |
| Number of pages | 6 |
| Journal | Yonsei medical journal |
| Volume | 54 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2013 Oct 31 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
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Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig. / Choi, Hong Kyu; Lee, Young Ho; Park, Jong Pil; Min, Kevin; Park, Hyojin.
In: Yonsei medical journal, Vol. 54, No. 6, 31.10.2013, p. 1336-1341.Research output: Contribution to journal › Article
TY - JOUR
T1 - Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig
AU - Choi, Hong Kyu
AU - Lee, Young Ho
AU - Park, Jong Pil
AU - Min, Kevin
AU - Park, Hyojin
PY - 2013/10/31
Y1 - 2013/10/31
N2 - Purpose: Small intestinal function returns first after surgery, and then the function of the stomach returns to normal after postoperative ileus (POI). The aim of this study was to investigate inflammatory responses in the muscle coat of stomach and small intestine in guinea pig POI model. Materials and Methods: The distance of charcoal migration from pylorus to the distal intestine was measured. Hematoxylin and eosin (H&E) and immunohistochemical stain for calprotectin were done from the histologic sections of stomach, jejunum and ileum obtained at 3 and 6 hour after operation. Data were compared between sham operation and POI groups. Results: The distance of charcoal migration was significantly reduced in the 3 and 6 hour POI groups compared with sham operated groups (p<0.05). On H&E staining, the degree of inflammation was significantly higher in the stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). Calprotectin positive cells were significantly increased in the muscle coat of stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). There was strong association between the degree of inflammation and calprotectin positive cells in stomach. Conclusion: Postoperative ileus induced by cecal manipulation significantly increased the degree of inflammation and calprotectin positive cells in the muscle coat of stomach as a remote organ. The relevance of degree of inflammation and the recovery time of ileus should be pursued in the future research.
AB - Purpose: Small intestinal function returns first after surgery, and then the function of the stomach returns to normal after postoperative ileus (POI). The aim of this study was to investigate inflammatory responses in the muscle coat of stomach and small intestine in guinea pig POI model. Materials and Methods: The distance of charcoal migration from pylorus to the distal intestine was measured. Hematoxylin and eosin (H&E) and immunohistochemical stain for calprotectin were done from the histologic sections of stomach, jejunum and ileum obtained at 3 and 6 hour after operation. Data were compared between sham operation and POI groups. Results: The distance of charcoal migration was significantly reduced in the 3 and 6 hour POI groups compared with sham operated groups (p<0.05). On H&E staining, the degree of inflammation was significantly higher in the stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). Calprotectin positive cells were significantly increased in the muscle coat of stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). There was strong association between the degree of inflammation and calprotectin positive cells in stomach. Conclusion: Postoperative ileus induced by cecal manipulation significantly increased the degree of inflammation and calprotectin positive cells in the muscle coat of stomach as a remote organ. The relevance of degree of inflammation and the recovery time of ileus should be pursued in the future research.
UR - http://www.scopus.com/inward/record.url?scp=84886566934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886566934&partnerID=8YFLogxK
U2 - 10.3349/ymj.2013.54.6.1336
DO - 10.3349/ymj.2013.54.6.1336
M3 - Article
C2 - 24142636
AN - SCOPUS:84886566934
VL - 54
SP - 1336
EP - 1341
JO - Yonsei Medical Journal
JF - Yonsei Medical Journal
SN - 0513-5796
IS - 6
ER -