Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig

Hong Kyu Choi, Young Ho Lee, Jong Pil Park, Kevin Min, HyoJin Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: Small intestinal function returns first after surgery, and then the function of the stomach returns to normal after postoperative ileus (POI). The aim of this study was to investigate inflammatory responses in the muscle coat of stomach and small intestine in guinea pig POI model. Materials and Methods: The distance of charcoal migration from pylorus to the distal intestine was measured. Hematoxylin and eosin (H&E) and immunohistochemical stain for calprotectin were done from the histologic sections of stomach, jejunum and ileum obtained at 3 and 6 hour after operation. Data were compared between sham operation and POI groups. Results: The distance of charcoal migration was significantly reduced in the 3 and 6 hour POI groups compared with sham operated groups (p<0.05). On H&E staining, the degree of inflammation was significantly higher in the stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). Calprotectin positive cells were significantly increased in the muscle coat of stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). There was strong association between the degree of inflammation and calprotectin positive cells in stomach. Conclusion: Postoperative ileus induced by cecal manipulation significantly increased the degree of inflammation and calprotectin positive cells in the muscle coat of stomach as a remote organ. The relevance of degree of inflammation and the recovery time of ileus should be pursued in the future research.

Original languageEnglish
Pages (from-to)1336-1341
Number of pages6
JournalYonsei medical journal
Volume54
Issue number6
DOIs
Publication statusPublished - 2013 Oct 31

Fingerprint

Ileus
Stomach
Guinea Pigs
Muscles
Leukocyte L1 Antigen Complex
Jejunum
Ileum
Inflammation
Charcoal
Pylorus
Hematoxylin
Eosine Yellowish-(YS)
Muscle Cells
Small Intestine
Intestines
Coloring Agents
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Choi, Hong Kyu ; Lee, Young Ho ; Park, Jong Pil ; Min, Kevin ; Park, HyoJin. / Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig. In: Yonsei medical journal. 2013 ; Vol. 54, No. 6. pp. 1336-1341.
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abstract = "Purpose: Small intestinal function returns first after surgery, and then the function of the stomach returns to normal after postoperative ileus (POI). The aim of this study was to investigate inflammatory responses in the muscle coat of stomach and small intestine in guinea pig POI model. Materials and Methods: The distance of charcoal migration from pylorus to the distal intestine was measured. Hematoxylin and eosin (H&E) and immunohistochemical stain for calprotectin were done from the histologic sections of stomach, jejunum and ileum obtained at 3 and 6 hour after operation. Data were compared between sham operation and POI groups. Results: The distance of charcoal migration was significantly reduced in the 3 and 6 hour POI groups compared with sham operated groups (p<0.05). On H&E staining, the degree of inflammation was significantly higher in the stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). Calprotectin positive cells were significantly increased in the muscle coat of stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). There was strong association between the degree of inflammation and calprotectin positive cells in stomach. Conclusion: Postoperative ileus induced by cecal manipulation significantly increased the degree of inflammation and calprotectin positive cells in the muscle coat of stomach as a remote organ. The relevance of degree of inflammation and the recovery time of ileus should be pursued in the future research.",
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Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig. / Choi, Hong Kyu; Lee, Young Ho; Park, Jong Pil; Min, Kevin; Park, HyoJin.

In: Yonsei medical journal, Vol. 54, No. 6, 31.10.2013, p. 1336-1341.

Research output: Contribution to journalArticle

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AU - Lee, Young Ho

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N2 - Purpose: Small intestinal function returns first after surgery, and then the function of the stomach returns to normal after postoperative ileus (POI). The aim of this study was to investigate inflammatory responses in the muscle coat of stomach and small intestine in guinea pig POI model. Materials and Methods: The distance of charcoal migration from pylorus to the distal intestine was measured. Hematoxylin and eosin (H&E) and immunohistochemical stain for calprotectin were done from the histologic sections of stomach, jejunum and ileum obtained at 3 and 6 hour after operation. Data were compared between sham operation and POI groups. Results: The distance of charcoal migration was significantly reduced in the 3 and 6 hour POI groups compared with sham operated groups (p<0.05). On H&E staining, the degree of inflammation was significantly higher in the stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). Calprotectin positive cells were significantly increased in the muscle coat of stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). There was strong association between the degree of inflammation and calprotectin positive cells in stomach. Conclusion: Postoperative ileus induced by cecal manipulation significantly increased the degree of inflammation and calprotectin positive cells in the muscle coat of stomach as a remote organ. The relevance of degree of inflammation and the recovery time of ileus should be pursued in the future research.

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