Influence of antifungal pre-treatment in preparing test mycelium on MIC values of several antifungal agents against Aspergillus niger

Jong Chul Park, Kosuke Takatori, Hun Jun Lee, Hideaki Matsuoka, Hiroshi Kurata

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1 Citation (Scopus)

Abstract

Antifungal activity of two imidazoles (miconazole and ketoconazole) and one polyene (amphotericin B) was evaluated using an automatic growth analysis system. Spores of Aspergillus niger were inoculated on the polylysine-coated glass bottom of a culture vessel. A colony formed in liquid medium was exposed to an antifungal agent and subsequently washed. Based on the dynamic growth rate of a test hypha selected from the colony in response to the antifungal agent, the minimum inhibitory concentration (MIC) was evaluated. The influence of time of reading (1, 2 and 3 h after washing) on the MIC determined was investigated. MICs for test hyphae subjected to antifungal pre-treatment were compared with those for hyphae without pre-treatment. Hyphae pre-treated with an antifungal agent for 1 h were found to become adapted and tolerant to that antifungal agent. Hyphae exposed and adapted to an imidazole obtained tolerance to amphotericin B as well as to the other imidazole.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalMycoscience
Volume37
Issue number2
DOIs
Publication statusPublished - 1996 Jul 15

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

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