Influence of naphthalene biodegradation on the adhesion of Pseudomonas putida NCIB 9816-4 to a naphthalene-contaminated soil

Geelsu Hwang, Sung Ryeol Park, Chang-Ha Lee, Ik Sung Ahn, Yeo Joon Yoon, Byung Jin Mhin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In an earlier study, Pseudomonas putida NCIB 9816-4, which was one of the most studied naphthalene-degrading bacteria, showed the preferred adhesion to the naphthalene-contaminated soil when it was in the exponential growth phase. The adhesion was found to take place through a hydrophobic interaction. We postulated that the surface hydrophobicity of P. putida NCIB 9816-4 in the exponential growth phase might be increased during the uptake of naphthalene, which caused the preferred adhesion to the naphthalene-contaminated soil. To verify this postulate, a plasmid-cured strain of P. putida NCIB 9816-4 was obtained in this study and compared with the wild-type for adhesion to the naphthalene-contaminated soil. Only the wild-type in the exponential growth phase showed increased adhesion to naphthalene-contaminated soil. The water contact angles of the two strains were measured in the presence and in the absence of naphthalene as indices of surface hydrophobicity. The water contact angle of the wild-type increased in the presence of naphthalene, whereas that of the cured strain did not change. We conclude that the uptake of naphthalene during naphthalene biodegradation in the exponential growth phase of P. putida NCIB 9816-4 made the cell surface more hydrophobic, resulting in increased adhesion to naphthalene-contaminated soil.

Original languageEnglish
Pages (from-to)491-493
Number of pages3
JournalJournal of Hazardous Materials
Volume172
Issue number1
DOIs
Publication statusPublished - 2009 Dec 15

Fingerprint

Pseudomonas putida
Naphthalene
Biodegradation
naphthalene
adhesion
biodegradation
Soil
Adhesion
Soils
Hydrophobic and Hydrophilic Interactions
hydrophobicity
Hydrophobicity
Growth
Contact angle
contaminated soil
Water
plasmid
Bacteria
Plasmids

All Science Journal Classification (ASJC) codes

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

Cite this

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abstract = "In an earlier study, Pseudomonas putida NCIB 9816-4, which was one of the most studied naphthalene-degrading bacteria, showed the preferred adhesion to the naphthalene-contaminated soil when it was in the exponential growth phase. The adhesion was found to take place through a hydrophobic interaction. We postulated that the surface hydrophobicity of P. putida NCIB 9816-4 in the exponential growth phase might be increased during the uptake of naphthalene, which caused the preferred adhesion to the naphthalene-contaminated soil. To verify this postulate, a plasmid-cured strain of P. putida NCIB 9816-4 was obtained in this study and compared with the wild-type for adhesion to the naphthalene-contaminated soil. Only the wild-type in the exponential growth phase showed increased adhesion to naphthalene-contaminated soil. The water contact angles of the two strains were measured in the presence and in the absence of naphthalene as indices of surface hydrophobicity. The water contact angle of the wild-type increased in the presence of naphthalene, whereas that of the cured strain did not change. We conclude that the uptake of naphthalene during naphthalene biodegradation in the exponential growth phase of P. putida NCIB 9816-4 made the cell surface more hydrophobic, resulting in increased adhesion to naphthalene-contaminated soil.",
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Influence of naphthalene biodegradation on the adhesion of Pseudomonas putida NCIB 9816-4 to a naphthalene-contaminated soil. / Hwang, Geelsu; Park, Sung Ryeol; Lee, Chang-Ha; Ahn, Ik Sung; Yoon, Yeo Joon; Mhin, Byung Jin.

In: Journal of Hazardous Materials, Vol. 172, No. 1, 15.12.2009, p. 491-493.

Research output: Contribution to journalArticle

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