Inhibition of Akt/FOXO3a signaling by constitutively active FOXO3a suppresses growth of follicular thyroid cancer cell lines

Zhen Yu Hong, Hyeon Jung Lee, Dong Yeob Shin, Suk Kyoung Kim, Mi Ran Seo, Eunjig Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Akt-dependent FOXO3a cytoplasmic translocation is an important tumorigenic mechanism for escaping from apoptosis in cancer cells. In the present study, we examined whether non-phosphorylatable FOXO3a can inhibit cell growth of various follicular thyroid carcinoma (FTC) cell lines. Adenovirus carrying the FOXO3a-triple mutant (TM) sequence including point mutations at three Akt phosphorylation sites (Ad-FOXO3a-TM) was generated and transduced to the cells to mimic inhibition of Akt/FOXO3a signal. Transduction of Ad-FOXO3a-TM to FTC133 cells induced cell cycle arrest and apoptosis. Injection of Ad-FOXO3a-TM suppressed the growth of xenograft tumors in athymic mice. Consequently, our results indicate that gene therapy based on Ad-FOXO3a-TM has therapeutic potential for FTC.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalCancer Letters
Volume314
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Follicular Adenocarcinoma
Cell Line
Growth
Apoptosis
Cell Cycle Checkpoints
Point Mutation
Heterografts
Adenoviridae
Nude Mice
Genetic Therapy
Neoplasms
Phosphorylation
Injections
Follicular Thyroid cancer
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Hong, Zhen Yu ; Lee, Hyeon Jung ; Shin, Dong Yeob ; Kim, Suk Kyoung ; Seo, Mi Ran ; Lee, Eunjig. / Inhibition of Akt/FOXO3a signaling by constitutively active FOXO3a suppresses growth of follicular thyroid cancer cell lines. In: Cancer Letters. 2012 ; Vol. 314, No. 1. pp. 34-40.
@article{b4c771f35f6a4954a414091739ca40cd,
title = "Inhibition of Akt/FOXO3a signaling by constitutively active FOXO3a suppresses growth of follicular thyroid cancer cell lines",
abstract = "Akt-dependent FOXO3a cytoplasmic translocation is an important tumorigenic mechanism for escaping from apoptosis in cancer cells. In the present study, we examined whether non-phosphorylatable FOXO3a can inhibit cell growth of various follicular thyroid carcinoma (FTC) cell lines. Adenovirus carrying the FOXO3a-triple mutant (TM) sequence including point mutations at three Akt phosphorylation sites (Ad-FOXO3a-TM) was generated and transduced to the cells to mimic inhibition of Akt/FOXO3a signal. Transduction of Ad-FOXO3a-TM to FTC133 cells induced cell cycle arrest and apoptosis. Injection of Ad-FOXO3a-TM suppressed the growth of xenograft tumors in athymic mice. Consequently, our results indicate that gene therapy based on Ad-FOXO3a-TM has therapeutic potential for FTC.",
author = "Hong, {Zhen Yu} and Lee, {Hyeon Jung} and Shin, {Dong Yeob} and Kim, {Suk Kyoung} and Seo, {Mi Ran} and Eunjig Lee",
year = "2012",
month = "1",
day = "1",
doi = "10.1016/j.canlet.2011.09.010",
language = "English",
volume = "314",
pages = "34--40",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Inhibition of Akt/FOXO3a signaling by constitutively active FOXO3a suppresses growth of follicular thyroid cancer cell lines. / Hong, Zhen Yu; Lee, Hyeon Jung; Shin, Dong Yeob; Kim, Suk Kyoung; Seo, Mi Ran; Lee, Eunjig.

In: Cancer Letters, Vol. 314, No. 1, 01.01.2012, p. 34-40.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibition of Akt/FOXO3a signaling by constitutively active FOXO3a suppresses growth of follicular thyroid cancer cell lines

AU - Hong, Zhen Yu

AU - Lee, Hyeon Jung

AU - Shin, Dong Yeob

AU - Kim, Suk Kyoung

AU - Seo, Mi Ran

AU - Lee, Eunjig

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Akt-dependent FOXO3a cytoplasmic translocation is an important tumorigenic mechanism for escaping from apoptosis in cancer cells. In the present study, we examined whether non-phosphorylatable FOXO3a can inhibit cell growth of various follicular thyroid carcinoma (FTC) cell lines. Adenovirus carrying the FOXO3a-triple mutant (TM) sequence including point mutations at three Akt phosphorylation sites (Ad-FOXO3a-TM) was generated and transduced to the cells to mimic inhibition of Akt/FOXO3a signal. Transduction of Ad-FOXO3a-TM to FTC133 cells induced cell cycle arrest and apoptosis. Injection of Ad-FOXO3a-TM suppressed the growth of xenograft tumors in athymic mice. Consequently, our results indicate that gene therapy based on Ad-FOXO3a-TM has therapeutic potential for FTC.

AB - Akt-dependent FOXO3a cytoplasmic translocation is an important tumorigenic mechanism for escaping from apoptosis in cancer cells. In the present study, we examined whether non-phosphorylatable FOXO3a can inhibit cell growth of various follicular thyroid carcinoma (FTC) cell lines. Adenovirus carrying the FOXO3a-triple mutant (TM) sequence including point mutations at three Akt phosphorylation sites (Ad-FOXO3a-TM) was generated and transduced to the cells to mimic inhibition of Akt/FOXO3a signal. Transduction of Ad-FOXO3a-TM to FTC133 cells induced cell cycle arrest and apoptosis. Injection of Ad-FOXO3a-TM suppressed the growth of xenograft tumors in athymic mice. Consequently, our results indicate that gene therapy based on Ad-FOXO3a-TM has therapeutic potential for FTC.

UR - http://www.scopus.com/inward/record.url?scp=82355169496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82355169496&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2011.09.010

DO - 10.1016/j.canlet.2011.09.010

M3 - Article

VL - 314

SP - 34

EP - 40

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -