Inhibition of apoptosis by taurine in macrophages treated with sodium nitroprusside

So Young Kim, Taesun Park, Ha Won Kim

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Abstract

Nitric oxide (NO) induces apoptotic cell death in murine RAW264.7 macrophages. To elucidate the mechanism underlying the inhibitory effect of taurine on NO-induced apoptosis, a cell was exposed to sodium nitroprusside (SNP), an NO donor, in the absence and presence of taurine. Taurine treatment prevented SNP-mediated cellular apoptosis in a concentration dependent manner. The exposure of the cell to taurine prior to SNP treatment inhibited DNA fragmentation more than addition of taurine to the medium after SNP treatment. Agarose gel electrophoresis data revealed that taurine reduced the intensity of SNP-induced DNA laddering. The taurine-mediated reduction in the number of apoptotic cells was also observed using the Hoechst 33258 stain. These results support the idea that taurine has the potential to function as an inhibitory modulator of NO-mediated cell injury.

Original languageEnglish
Title of host publicationTaurine 7
EditorsJunichi Azuma, Ito Takashi, Stephen Schaffer
Pages481-489
Number of pages9
DOIs
Publication statusPublished - 2009 Apr 22

Publication series

NameAdvances in Experimental Medicine and Biology
Volume643
ISSN (Print)0065-2598

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kim, S. Y., Park, T., & Kim, H. W. (2009). Inhibition of apoptosis by taurine in macrophages treated with sodium nitroprusside. In J. Azuma, I. Takashi, & S. Schaffer (Eds.), Taurine 7 (pp. 481-489). (Advances in Experimental Medicine and Biology; Vol. 643). https://doi.org/10.1007/978-0-387-75681-3_50