Inhibition of connexin 43 alters Shh and Bmp-2 expression patterns in embryonic mouse tongue

Jae Young Kim, Sung Won Cho, Min Jung Lee, Heui Jung Hwang, Jong Min Lee, Syng Ill Lee, Takashi Muramatsu, Masaki Shimono, Han Sung Jung

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The morphogenesis of fungiform papillae occurs in a stereotyped pattern on the dorsal surface of the mammalian tongue via epithelial-mesenchymal interactions. These interactions are thought to be achieved via intercellular communication. Gap junctions can be observed in many developing tissues and have been suggested to participate in a variety of functions, including the regulation of cell proliferation, differentiation, and apoptosis. Here, we demonstrate that the expression of Connexin 43 (Cx43), a gap junction protein, is correlated significantly with the development of fungiform papillae, which exhibit a pattern formation and morphogenesis similar to the development of other epithelial appendages. Antisense-oligodeoxynucleotide (AS-ODN) against Cx43 was used to assess the developmental functions of Cx43. The expression patterns of the signaling molecules were disrupted by Cx43 inhibition. Interestingly, the expression patterns of Shh, a key molecule in the determination of the spacing patterns of fungiform papillae, were disturbed after treatment with Cx43 AS-ODN. We have also attempted to determine the functions of Bmp-2 by applying NOGGIN protein to tongue cultures. Our results indicate that upstream regulation via Cx43 controls the Shh and Bmp-2 pathways for the morphogenesis and pattern formation of fungiform papillae.

Original languageEnglish
Pages (from-to)409-415
Number of pages7
JournalCell and Tissue Research
Volume320
Issue number3
DOIs
Publication statusPublished - 2005 Jun 1

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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    Kim, J. Y., Cho, S. W., Lee, M. J., Hwang, H. J., Lee, J. M., Lee, S. I., Muramatsu, T., Shimono, M., & Jung, H. S. (2005). Inhibition of connexin 43 alters Shh and Bmp-2 expression patterns in embryonic mouse tongue. Cell and Tissue Research, 320(3), 409-415. https://doi.org/10.1007/s00441-005-1091-y