Inhibition of histone deacetylase1 induces autophagy

Meeyeon Oh, In Kwon Choi, Ho Jeong Kwon

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Autophagy is a process where cytoplasmic materials are degraded by lysosomal machinery. Histone deacetylase (HDAC) inhibitors induce autophagy, and HDAC6, one of class II HDAC isotypes, is directly involved in autophagic degradation in the cell. However, it is unclear if class I HDAC isotype such as HDCA1 is involved in this process. To investigate if class I HDAC isotype is involved in autophagy, a specific class I HDAC inhibitor and an siRNA of HDAC1 were used to treat HeLa cells. Autophagic markers were then investigated. Both inhibition and genetic knock-down of HDAC1 in the cells significantly induced autophagic vacuole formation and lysosome function. Moreover, disruption of HDAC1 leads to the conversion of LC3-I to LC3-II. Together, these results demonstrate that HDAC1 could play a role in autophagy and specific inhibition of HDAC1 can induce autophagy.

Original languageEnglish
Pages (from-to)1179-1183
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume369
Issue number4
DOIs
Publication statusPublished - 2008 May 16

Fingerprint

Histone Deacetylases
Autophagy
Histones
Histone Deacetylase Inhibitors
Small Interfering RNA
Machinery
Degradation
Vacuoles
Lysosomes
HeLa Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "Autophagy is a process where cytoplasmic materials are degraded by lysosomal machinery. Histone deacetylase (HDAC) inhibitors induce autophagy, and HDAC6, one of class II HDAC isotypes, is directly involved in autophagic degradation in the cell. However, it is unclear if class I HDAC isotype such as HDCA1 is involved in this process. To investigate if class I HDAC isotype is involved in autophagy, a specific class I HDAC inhibitor and an siRNA of HDAC1 were used to treat HeLa cells. Autophagic markers were then investigated. Both inhibition and genetic knock-down of HDAC1 in the cells significantly induced autophagic vacuole formation and lysosome function. Moreover, disruption of HDAC1 leads to the conversion of LC3-I to LC3-II. Together, these results demonstrate that HDAC1 could play a role in autophagy and specific inhibition of HDAC1 can induce autophagy.",
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Inhibition of histone deacetylase1 induces autophagy. / Oh, Meeyeon; Choi, In Kwon; Kwon, Ho Jeong.

In: Biochemical and Biophysical Research Communications, Vol. 369, No. 4, 16.05.2008, p. 1179-1183.

Research output: Contribution to journalArticle

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