Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8

Ho Jin Sung, Sooho Choi, Ji Won Lee, Chang Youp Ok, Yoe Sik Bae, Yun Hee Kim, Weontae Lee, Kyun Heo, In Hoo Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that has been associated with the promotion of neutrophil chemotaxis, degranulation, and the pathogenesis of several neutrophil-infiltrating chronic inflammatory diseases. In the current study, we generated and characterized a 2'-fluoro-pyrimidine modified RNA aptamer (8A-35) against human IL-8. The 8A-35 aptamer binds to IL-8 with high specificity and affinity, yielding an estimated KD of 1.72pM. NMR data revealed that the residues of Lys8, Leu10, Val63, Val66, Lys69 and Ala74 of IL-8 interact with aptamer. Moreover, the 8A-35 aptamer has a potent IL-8-neutralizing activity that can modulate multiple biological activities of IL-8 in human neutrophils, including migration, intracellular signaling, and intracellular Ca2+ mobilization. Our results suggest that the 8A-35 aptamer has great potential to be a lead structure in the development of effective therapeutic agents against inflammatory diseases.

Original languageEnglish
Pages (from-to)578-589
Number of pages12
JournalBiomaterials
Volume35
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Fingerprint Dive into the research topics of 'Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8'. Together they form a unique fingerprint.

  • Cite this

    Sung, H. J., Choi, S., Lee, J. W., Ok, C. Y., Bae, Y. S., Kim, Y. H., Lee, W., Heo, K., & Kim, I. H. (2014). Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8. Biomaterials, 35(1), 578-589. https://doi.org/10.1016/j.biomaterials.2013.09.107