Inhibition of invasion and capillary-like tube formation by retrohydroxamate-based MMP inhibitors

Seung Su Choi, Ae Ri Ji, Seung Woo Yu, Bong Hwan Cho, Jung Dae Park, Jun Hyoung Park, Hyun Soo Lee, Seong Eon Ryu, Dong Han Kim, Jae Hoon Kang, Seung Taek Lee

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Abstract

Matrix metalloproteinases (MMPs), a family of zinc-containing endopeptidases, participate in many normal processes such as embryonic development and wound repair, and in many pathological situations such as cancer, atherosclerosis, and arthritis. Peptidomimetic MMP inhibitors were designed and synthesized with Nformylhydroxylamine (retrohydroxamate) as a zinc-binding group and various side chains on the α, P1′, and P2′ positions. Using in vitro MMP assays with purified MMPs (MMP-1, MMP-2, MMP-3, MMP-9, and MMP-14) and fluorogenic peptide substrates, it was found that compounds 2d and 2g selectively inhibit gelatinases (MMP-2 and MMP-9) and interstitial collagenase (MMP-1). They also inhibited the chemo-invasion of fibrosarcoma HT-1080 cells and tube formation of human umbilical vascular endothelial cells in a dosedependent manner. Our results suggest that retrohydroxamate-based MMP inhibitors, especially compounds 2d and 2g, have the potential to be used as therapeutic drugs for cancer and other MMP-related diseases.

Original languageEnglish
Pages (from-to)2032-2038
Number of pages7
JournalBulletin of the Korean Chemical Society
Volume32
Issue number6
DOIs
Publication statusPublished - 2011 Jun 20

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Choi, S. S., Ji, A. R., Yu, S. W., Cho, B. H., Park, J. D., Park, J. H., Lee, H. S., Ryu, S. E., Kim, D. H., Kang, J. H., & Lee, S. T. (2011). Inhibition of invasion and capillary-like tube formation by retrohydroxamate-based MMP inhibitors. Bulletin of the Korean Chemical Society, 32(6), 2032-2038. https://doi.org/10.5012/bkcs.2011.32.6.2032