Inhibition of miR-449a Promotes Cartilage Regeneration and Prevents Progression of Osteoarthritis in In Vivo Rat Models

Dawoon Baek, Kyoung Mi Lee, Ki Won Park, Jae Wan Suh, Seong Mi Choi, Kwang Hwan Park, Jin Woo Lee, Sung Hwan Kim

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Traumatic and degenerative lesions of articular cartilage usually progress to osteoarthritis (OA), a leading cause of disability in humans. MicroRNAs (miRNAs) can regulate the differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) and play important roles in the expression of genes related to OA. However, their functional roles in OA remain poorly understood. Here, we have examined miR-449a, which targets sirtuin 1 (SIRT1) and lymphoid enhancer-binding factor-1 (LEF-1), and observed its effects on damaged cartilage. The levels of chondrogenic markers and miR-449a target genes increased during chondrogenesis in anti-miR-449a-transfected hBMSCs. A locked nucleic acid (LNA)-anti-miR-449a increased cartilage regeneration and expression of type II collagen and aggrecan on the regenerated cartilage surface in acute defect and OA models. Furthermore, intra-articular injection of LNA-anti-miR-449a prevented disease progression in the OA model. Our study indicates that miR-449a may be a novel potential therapeutic target for age-related joint diseases like OA.

Original languageEnglish
Pages (from-to)322-333
Number of pages12
JournalMolecular Therapy - Nucleic Acids
Volume13
DOIs
Publication statusPublished - 2018 Dec 7

Bibliographical note

Funding Information:
This work was supported by grants from the Ministry of Science of Republic of Korea (grant NRF-2014R1A1A1007983 ) and by the Mid-career Researcher Program through an NRF grant funded by the MEST ( NRF-2018R1A2B6007376 ).

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery

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