Inhibition of mitogen-activated protein kinase by a Drosophila dual-specific phosphatase

Won Jae Lee, Sun Hong Kim, Yong Sik Kim, Sung Jun Han, Ki Sook Park, Ji Hwan Ryu, Man Wook Hur, Kang-Yell Choi

Research output: Contribution to journalArticle

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Abstract

The Drosophila extracellular signal-regulated kinase (DERK) mitogen-activated protein kinase (MAPK) is involved in the regulation of multiple differentiation and developmental processes. Tight control of MAPK activity is critical for normal cell behaviour. We identified a novel Drosophila MAPK phosphatase (DMKP) cDNA from the expressed-sequence-tag database and characterized it. Analysis of the nucleotide sequence revealed an open reading frame encoding the 203-amino acid protein, with a calculated molecular mass of 23 kDa, which has a high amino acid sequence similarity with 'VH1-like' dual-specific phosphatases at the broad region near the catalytic sites. The expression of DMKP mRNA occurs from the late larval stages to adulthood in Drosophila development. The recombinant DMKP protein produced in yeast retained its phosphatase activity. When expressed in Schneider cells, DMKP dose dependently inhibited DERK and Drosophila c-Jun N-terminal kinase activities with high selectivity towards DERK. However, DMKP did not have any affect on Drosophila p38 activity. When DMKP was expressed in yeast, it down-regulated the fus1-lacZ trans-reporter gene of the pheromone MAPK pathway without any significant effect on the high-osmolarity-glycerol-response pathway.

Original languageEnglish
Pages (from-to)821-828
Number of pages8
JournalBiochemical Journal
Volume349
Issue number3
DOIs
Publication statusPublished - 2000 Aug 1

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Mitogen-Activated Protein Kinases
Phosphoric Monoester Hydrolases
Drosophila
Extracellular Signal-Regulated MAP Kinases
Yeast
Mitogen-Activated Protein Kinase Phosphatases
Amino Acids
JNK Mitogen-Activated Protein Kinases
Pheromones
Expressed Sequence Tags
Yeasts
Molecular mass
Glycerol
Proteins
Nucleotides
Complementary DNA
Genes
Inhibition (Psychology)
Drosophila Dmel-MKP protein
Reporter Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lee, Won Jae ; Kim, Sun Hong ; Kim, Yong Sik ; Han, Sung Jun ; Park, Ki Sook ; Ryu, Ji Hwan ; Hur, Man Wook ; Choi, Kang-Yell. / Inhibition of mitogen-activated protein kinase by a Drosophila dual-specific phosphatase. In: Biochemical Journal. 2000 ; Vol. 349, No. 3. pp. 821-828.
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abstract = "The Drosophila extracellular signal-regulated kinase (DERK) mitogen-activated protein kinase (MAPK) is involved in the regulation of multiple differentiation and developmental processes. Tight control of MAPK activity is critical for normal cell behaviour. We identified a novel Drosophila MAPK phosphatase (DMKP) cDNA from the expressed-sequence-tag database and characterized it. Analysis of the nucleotide sequence revealed an open reading frame encoding the 203-amino acid protein, with a calculated molecular mass of 23 kDa, which has a high amino acid sequence similarity with 'VH1-like' dual-specific phosphatases at the broad region near the catalytic sites. The expression of DMKP mRNA occurs from the late larval stages to adulthood in Drosophila development. The recombinant DMKP protein produced in yeast retained its phosphatase activity. When expressed in Schneider cells, DMKP dose dependently inhibited DERK and Drosophila c-Jun N-terminal kinase activities with high selectivity towards DERK. However, DMKP did not have any affect on Drosophila p38 activity. When DMKP was expressed in yeast, it down-regulated the fus1-lacZ trans-reporter gene of the pheromone MAPK pathway without any significant effect on the high-osmolarity-glycerol-response pathway.",
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Lee, WJ, Kim, SH, Kim, YS, Han, SJ, Park, KS, Ryu, JH, Hur, MW & Choi, K-Y 2000, 'Inhibition of mitogen-activated protein kinase by a Drosophila dual-specific phosphatase', Biochemical Journal, vol. 349, no. 3, pp. 821-828. https://doi.org/10.1042/bj3490821

Inhibition of mitogen-activated protein kinase by a Drosophila dual-specific phosphatase. / Lee, Won Jae; Kim, Sun Hong; Kim, Yong Sik; Han, Sung Jun; Park, Ki Sook; Ryu, Ji Hwan; Hur, Man Wook; Choi, Kang-Yell.

In: Biochemical Journal, Vol. 349, No. 3, 01.08.2000, p. 821-828.

Research output: Contribution to journalArticle

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