Inhibition of the Nav1.7 Channel in the Trigeminal Ganglion Relieves Pulpitis Inflammatory Pain

Minjee Kwon, Il Young Jung, Myeounghoon Cha, Bae Hwan Lee

Research output: Contribution to journalArticlepeer-review


Pulpitis causes significant changes in the peripheral nervous system, which induce hyperalgesia. However, the relationship between neuronal activity and Nav1.7 expression following pulpal noxious pain has not yet been investigated in the trigeminal ganglion (TG). The aim of our study was to verify whether experimentally induced pulpitis activates the expression of Nav1.7 peripherally and the neuronal activities of the TGs can be affected by Nav1.7 channel inhibition. Acute pulpitis was induced through allyl isothiocyanate (AITC) application to the rat maxillary molar tooth pulp. Three days after AITC application, abnormal pain behaviors were recorded, and the rats were euthanized to allow for immunohistochemical, optical imaging, and western blot analyses of the Nav1.7 expression in the TG. A significant increase in AITC-induced pain-like behaviors and histological evidence of pulpitis were observed. In addition, histological and western blot data showed that Nav1.7 expressions in the TGs were significantly higher in the AITC group than in the naive and saline group rats. Optical imaging showed that the AITC group showed higher neuronal activity after electrical stimulation of the TGs. Additionally, treatment of ProTxII, selective Nav1.7 blocker, on to the TGs in the AITC group effectively suppressed the hyperpolarized activity after electrical stimulation. These findings indicate that the inhibition of the Nav1.7 channel could modulate nociceptive signal processing in the TG following pulp inflammation.

Original languageEnglish
Article number759730
JournalFrontiers in Pharmacology
Publication statusPublished - 2021 Dec 8

Bibliographical note

Funding Information:
This study was supported by the Basic Research Program through the National Research Foundation (NRF) funded by the Ministry of Science and ICT and Future Planning (NRF-2019R1I1A1A01059697 and 2020R1A2C3008481).

Publisher Copyright:
Copyright © 2021 Kwon, Jung, Cha and Lee.

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)


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