Inhibitory effect of 5-hydroxymethylfurfural on continuous hydrogen fermentation by mixed culture in a fixed bed reactor

Parthiban Anburajan, Arivalagan Pugazhendhi, Jong Hun Park, Gopalakrishnan Kumar, Chang Su Choi, Sang Hyoun Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The authors explored the inhibitory effect of 5-hydroxymethylfurfural (5-HMF) on continuous hydrogen fermentation using a mixed microbial population. A lab-scale fixed bed reactor (FBR) with a 3.0-L working volume was fed with 15 g/L of glucose as sole carbon source at 6 h HRT, while the 5-HMF concentration in feedstock was increased stepwise from 1.2 to 2.7 g/L. 5-HMF gradually decreased hydrogen production. At 2.7 g 5-HMF/L, hydrogen yield, and hydrogen production rate were 0.86 ± 0.08 mol H2/mol glucoseadded and 7.4 L H2/L-d, respectively, which were 15% of the yield and production rate observed without 5-HMF. Quantitative polymerase chain reaction (qPCR) analysis showed that 5-HMF changed the dominant bacteria from Clostridium sp. to Lactobacillus sp. Scanning electronic microscope (SEM) showed the biofilm structure of mixed culture was also damaged by 5-HMF. However, the inhibition was milder than that on batch fermentation reported earlier. Furthermore, the H2-producing performance, bacterial population, and granular morphology were partially recovered when 5-HMF was removed. The resistance to 5-HMF inhibition would result from the acclimation during continuous operation and attached growth microorganism by immobilization and entrapment.

Original languageEnglish
Pages (from-to)27570-27576
Number of pages7
JournalInternational Journal of Hydrogen Energy
Volume42
Issue number45
DOIs
Publication statusPublished - 2017 Jan 1

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fermentation
hydrogen production
Hydrogen production
Fermentation
beds
reactors
Clostridium
Enzyme inhibition
polymerase chain reaction
Hydrogen
biofilms
entrapment
Polymerase chain reaction
Biofilms
microorganisms
hydrogen
immobilization
glucose
Microorganisms
Feedstocks

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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title = "Inhibitory effect of 5-hydroxymethylfurfural on continuous hydrogen fermentation by mixed culture in a fixed bed reactor",
abstract = "The authors explored the inhibitory effect of 5-hydroxymethylfurfural (5-HMF) on continuous hydrogen fermentation using a mixed microbial population. A lab-scale fixed bed reactor (FBR) with a 3.0-L working volume was fed with 15 g/L of glucose as sole carbon source at 6 h HRT, while the 5-HMF concentration in feedstock was increased stepwise from 1.2 to 2.7 g/L. 5-HMF gradually decreased hydrogen production. At 2.7 g 5-HMF/L, hydrogen yield, and hydrogen production rate were 0.86 ± 0.08 mol H2/mol glucoseadded and 7.4 L H2/L-d, respectively, which were 15{\%} of the yield and production rate observed without 5-HMF. Quantitative polymerase chain reaction (qPCR) analysis showed that 5-HMF changed the dominant bacteria from Clostridium sp. to Lactobacillus sp. Scanning electronic microscope (SEM) showed the biofilm structure of mixed culture was also damaged by 5-HMF. However, the inhibition was milder than that on batch fermentation reported earlier. Furthermore, the H2-producing performance, bacterial population, and granular morphology were partially recovered when 5-HMF was removed. The resistance to 5-HMF inhibition would result from the acclimation during continuous operation and attached growth microorganism by immobilization and entrapment.",
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Inhibitory effect of 5-hydroxymethylfurfural on continuous hydrogen fermentation by mixed culture in a fixed bed reactor. / Anburajan, Parthiban; Pugazhendhi, Arivalagan; Park, Jong Hun; Kumar, Gopalakrishnan; Choi, Chang Su; Kim, Sang Hyoun.

In: International Journal of Hydrogen Energy, Vol. 42, No. 45, 01.01.2017, p. 27570-27576.

Research output: Contribution to journalArticle

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AU - Anburajan, Parthiban

AU - Pugazhendhi, Arivalagan

AU - Park, Jong Hun

AU - Kumar, Gopalakrishnan

AU - Choi, Chang Su

AU - Kim, Sang Hyoun

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