Inhibitory effect of angiotensin II receptor antagonist on the contraction and growth of hepatic stellate cells

Soonkoo Baik, Ho Sung Jo, Ki Tae Suk, Jung Min Kim, Byong Jun Lee, Yeun Jong Choi, Hyunsoo Kim, DongKi Lee, Sang Ok Kwon, Keon Il Lee, Seungkuy Cha, Kyusang Park, Indeok Kong

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Abstract

BACKGROUND/AIMS: This study aimed to investigate the effects of angiotensin II (ANG II) and its receptor antagonist (losartan) on the contraction and growth of HSCs. METHODS: HSCs were isolated from Sprague Dawley rat and cultured at various conditions as follows: control, pretreatment of 10(-5) M ANG II, pretreatment of 10(-5) M endothelin, and pretreatment of 10(-5) M ANG II and 10(-6) M losartan. We conducted morphologic analysis with cellular area and length by image analysis system to estimate cell growth in each group. In addition, we measured the change of intracellular calcium currents via electrophysiological methods to evaluate the contractile effect of ANG II and losartan on HSCs. RESULTS: At the fifth day of incubation, the mean cellular area of ANG II-pretreated group and ANG II with losartan-pretreated group were 704.68+/-22.6 micro m2 and 332.90+/-32.6 micro m2, respectively. This difference was statistically significant (p<0.05). ANG II induced an increase in the intracellular calcium current by 22.0+/-3.0% compared with basal current level (p<0.05). However, when losartan was pretreated, ANG II did not cause a significant increase in calcium current (3.1+/-0.8%, p>0.05). CONCLUSION: ANG II accelerates the contraction and growth of HSCs, while its receptor blocker, losartan, inhibits the contraction and growth of HSCs.

Original languageEnglish
Pages (from-to)134-141
Number of pages8
JournalThe Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi
Volume42
Issue number2
Publication statusPublished - 2003 Jan 1

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Hepatic Stellate Cells
Angiotensin Receptor Antagonists
Losartan
Angiotensin II
Growth
Endothelins
Sprague Dawley Rats
Calcium

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Inhibitory effect of angiotensin II receptor antagonist on the contraction and growth of hepatic stellate cells",
abstract = "BACKGROUND/AIMS: This study aimed to investigate the effects of angiotensin II (ANG II) and its receptor antagonist (losartan) on the contraction and growth of HSCs. METHODS: HSCs were isolated from Sprague Dawley rat and cultured at various conditions as follows: control, pretreatment of 10(-5) M ANG II, pretreatment of 10(-5) M endothelin, and pretreatment of 10(-5) M ANG II and 10(-6) M losartan. We conducted morphologic analysis with cellular area and length by image analysis system to estimate cell growth in each group. In addition, we measured the change of intracellular calcium currents via electrophysiological methods to evaluate the contractile effect of ANG II and losartan on HSCs. RESULTS: At the fifth day of incubation, the mean cellular area of ANG II-pretreated group and ANG II with losartan-pretreated group were 704.68+/-22.6 micro m2 and 332.90+/-32.6 micro m2, respectively. This difference was statistically significant (p<0.05). ANG II induced an increase in the intracellular calcium current by 22.0+/-3.0{\%} compared with basal current level (p<0.05). However, when losartan was pretreated, ANG II did not cause a significant increase in calcium current (3.1+/-0.8{\%}, p>0.05). CONCLUSION: ANG II accelerates the contraction and growth of HSCs, while its receptor blocker, losartan, inhibits the contraction and growth of HSCs.",
author = "Soonkoo Baik and Jo, {Ho Sung} and Suk, {Ki Tae} and Kim, {Jung Min} and Lee, {Byong Jun} and Choi, {Yeun Jong} and Hyunsoo Kim and DongKi Lee and Kwon, {Sang Ok} and Lee, {Keon Il} and Seungkuy Cha and Kyusang Park and Indeok Kong",
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Inhibitory effect of angiotensin II receptor antagonist on the contraction and growth of hepatic stellate cells. / Baik, Soonkoo; Jo, Ho Sung; Suk, Ki Tae; Kim, Jung Min; Lee, Byong Jun; Choi, Yeun Jong; Kim, Hyunsoo; Lee, DongKi; Kwon, Sang Ok; Lee, Keon Il; Cha, Seungkuy; Park, Kyusang; Kong, Indeok.

In: The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi, Vol. 42, No. 2, 01.01.2003, p. 134-141.

Research output: Contribution to journalArticle

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T1 - Inhibitory effect of angiotensin II receptor antagonist on the contraction and growth of hepatic stellate cells

AU - Baik, Soonkoo

AU - Jo, Ho Sung

AU - Suk, Ki Tae

AU - Kim, Jung Min

AU - Lee, Byong Jun

AU - Choi, Yeun Jong

AU - Kim, Hyunsoo

AU - Lee, DongKi

AU - Kwon, Sang Ok

AU - Lee, Keon Il

AU - Cha, Seungkuy

AU - Park, Kyusang

AU - Kong, Indeok

PY - 2003/1/1

Y1 - 2003/1/1

N2 - BACKGROUND/AIMS: This study aimed to investigate the effects of angiotensin II (ANG II) and its receptor antagonist (losartan) on the contraction and growth of HSCs. METHODS: HSCs were isolated from Sprague Dawley rat and cultured at various conditions as follows: control, pretreatment of 10(-5) M ANG II, pretreatment of 10(-5) M endothelin, and pretreatment of 10(-5) M ANG II and 10(-6) M losartan. We conducted morphologic analysis with cellular area and length by image analysis system to estimate cell growth in each group. In addition, we measured the change of intracellular calcium currents via electrophysiological methods to evaluate the contractile effect of ANG II and losartan on HSCs. RESULTS: At the fifth day of incubation, the mean cellular area of ANG II-pretreated group and ANG II with losartan-pretreated group were 704.68+/-22.6 micro m2 and 332.90+/-32.6 micro m2, respectively. This difference was statistically significant (p<0.05). ANG II induced an increase in the intracellular calcium current by 22.0+/-3.0% compared with basal current level (p<0.05). However, when losartan was pretreated, ANG II did not cause a significant increase in calcium current (3.1+/-0.8%, p>0.05). CONCLUSION: ANG II accelerates the contraction and growth of HSCs, while its receptor blocker, losartan, inhibits the contraction and growth of HSCs.

AB - BACKGROUND/AIMS: This study aimed to investigate the effects of angiotensin II (ANG II) and its receptor antagonist (losartan) on the contraction and growth of HSCs. METHODS: HSCs were isolated from Sprague Dawley rat and cultured at various conditions as follows: control, pretreatment of 10(-5) M ANG II, pretreatment of 10(-5) M endothelin, and pretreatment of 10(-5) M ANG II and 10(-6) M losartan. We conducted morphologic analysis with cellular area and length by image analysis system to estimate cell growth in each group. In addition, we measured the change of intracellular calcium currents via electrophysiological methods to evaluate the contractile effect of ANG II and losartan on HSCs. RESULTS: At the fifth day of incubation, the mean cellular area of ANG II-pretreated group and ANG II with losartan-pretreated group were 704.68+/-22.6 micro m2 and 332.90+/-32.6 micro m2, respectively. This difference was statistically significant (p<0.05). ANG II induced an increase in the intracellular calcium current by 22.0+/-3.0% compared with basal current level (p<0.05). However, when losartan was pretreated, ANG II did not cause a significant increase in calcium current (3.1+/-0.8%, p>0.05). CONCLUSION: ANG II accelerates the contraction and growth of HSCs, while its receptor blocker, losartan, inhibits the contraction and growth of HSCs.

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