Consumption of fruits and vegetables is correlated with a lower incidence of cancer. Here, we identified hyperoside as an active compound from Acanthopanax sessiliflorus, and investigated the effect of hyperoside on UVB-induced transactivation of activator protein 1 (AP-1) and on the mitogen-activated protein kinase signalling pathway in JB6 P + cells. Hyperoside inhibited UVB-induced AP-1 transactivation. It inhibited the UVB-induced phosphorylation of p90RSK. Kinase assays revealed that hyperoside significantly inhibited ERK1/2 activity. Furthermore, hyperoside bound to ERK1/2 to suppress its activity. In addition, phosphorylation of cAMP response element binding protein (CREB) and signal transducers and activators of transcription (STAT) 3 were suppressed by hyperoside. Overall, these results indicate that hyperoside may be a promising chemopreventive agent that acts by suppressing the transactivation of AP-1 and the phosphorylation of p90RSK, CREB, and STAT3 through the binding and inhibition of ERK1/2.
Bibliographical noteFunding Information:
This work was supported by grants from World Class University Program ( R31-2008-00-10056-0 ), World Class Institute Program ( 2009-002 ), Leap Research Program ( 2010-0029233 ), National Research Foundation of Korea Grant ( NRF-2009-352-F00043 ), and Technology Development Program for Agriculture and Forestry ( 107055-2 ), Republic of Korea .
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Food Science