TY - JOUR
T1 - Inhibitory effect of ethyl acetate extract of white peach pericarp on adipogenesis of 3T3-L1 preadipocyte cells
AU - Park, Hong Gyu
AU - Kim, Jin Moon
AU - Kim, Jung Mogg
AU - Chung, Won Yoon
AU - Yoo, Yun Jung
AU - Cha, Jeong Heon
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2008
Y1 - 2008
N2 - In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.
AB - In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.
UR - http://www.scopus.com/inward/record.url?scp=61349093574&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61349093574&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:61349093574
VL - 17
SP - 1327
EP - 1331
JO - Food Science and Biotechnology
JF - Food Science and Biotechnology
SN - 1226-7708
IS - 6
ER -