Abstract
In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 1327-1331 |
| Number of pages | 5 |
| Journal | Food Science and Biotechnology |
| Volume | 17 |
| Issue number | 6 |
| Publication status | Published - 2008 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
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Inhibitory effect of ethyl acetate extract of white peach pericarp on adipogenesis of 3T3-L1 preadipocyte cells. / Park, Hong Gyu; Kim, Jin Moon; Kim, Jung Mogg; Chung, Won Yoon; Yoo, Yun Jung; Cha, Jeong Heon.
In: Food Science and Biotechnology, Vol. 17, No. 6, 01.12.2008, p. 1327-1331.Research output: Contribution to journal › Article
TY - JOUR
T1 - Inhibitory effect of ethyl acetate extract of white peach pericarp on adipogenesis of 3T3-L1 preadipocyte cells
AU - Park, Hong Gyu
AU - Kim, Jin Moon
AU - Kim, Jung Mogg
AU - Chung, Won Yoon
AU - Yoo, Yun Jung
AU - Cha, Jeong Heon
PY - 2008/12/1
Y1 - 2008/12/1
N2 - In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.
AB - In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.
UR - http://www.scopus.com/inward/record.url?scp=61349093574&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61349093574&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:61349093574
VL - 17
SP - 1327
EP - 1331
JO - Food Science and Biotechnology
JF - Food Science and Biotechnology
SN - 1226-7708
IS - 6
ER -