Inhibitory effect of ethyl acetate extract of white peach pericarp on adipogenesis of 3T3-L1 preadipocyte cells

Hong Gyu Park, Jin Moon Kim, Jung Mogg Kim, Won Yoon Chung, Yun Jung Yoo, Jeong Heon Cha

Research output: Contribution to journalArticle

Abstract

In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.

Original languageEnglish
Pages (from-to)1327-1331
Number of pages5
JournalFood Science and Biotechnology
Volume17
Issue number6
Publication statusPublished - 2008 Dec 1

Fingerprint

3T3-L1 Cells
Adipogenesis
pericarp
adipocytes
Adipocytes
peaches
extracts
cells
lipids
CCAAT-Enhancer-Binding Proteins
Lipids
fatty acid-binding proteins
Fatty Acid-Binding Proteins
1-Butanol
Peroxisome Proliferator-Activated Receptors
butanol
droplets
dyes
binding proteins
Coloring Agents

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

@article{e565704c383a4c8aa922df634e061328,
title = "Inhibitory effect of ethyl acetate extract of white peach pericarp on adipogenesis of 3T3-L1 preadipocyte cells",
abstract = "In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.",
author = "Park, {Hong Gyu} and Kim, {Jin Moon} and Kim, {Jung Mogg} and Chung, {Won Yoon} and Yoo, {Yun Jung} and Cha, {Jeong Heon}",
year = "2008",
month = "12",
day = "1",
language = "English",
volume = "17",
pages = "1327--1331",
journal = "Food Science and Biotechnology",
issn = "1226-7708",
publisher = "Springer Netherlands",
number = "6",

}

Inhibitory effect of ethyl acetate extract of white peach pericarp on adipogenesis of 3T3-L1 preadipocyte cells. / Park, Hong Gyu; Kim, Jin Moon; Kim, Jung Mogg; Chung, Won Yoon; Yoo, Yun Jung; Cha, Jeong Heon.

In: Food Science and Biotechnology, Vol. 17, No. 6, 01.12.2008, p. 1327-1331.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibitory effect of ethyl acetate extract of white peach pericarp on adipogenesis of 3T3-L1 preadipocyte cells

AU - Park, Hong Gyu

AU - Kim, Jin Moon

AU - Kim, Jung Mogg

AU - Chung, Won Yoon

AU - Yoo, Yun Jung

AU - Cha, Jeong Heon

PY - 2008/12/1

Y1 - 2008/12/1

N2 - In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.

AB - In order to determine whether peach contains compounds to regulate adipocyte differentiation, extracts of flesh/ pericarp of yellow/white peach were prepared in water, ethyl acetate (EtOAc), or n-butanol solvent and determined for effects on adipocyte differentiation in C3H10T1/2 or 3T3-L1 cells. Interestingly, none of peach extracts has statistically significant stimulatory effect on the adipocyte differentiation in C3H10T1/2. Furthermore, the presence of EtOAc extract of white peach pericarp (WPP) was found to inhibit lipid accumulation in 3T3-L1 cells both by microscopic examination of Oil Red O-stained lipid droplets and by spectrophotometric quantification of extracted stain, indicating a significant inhibitory effect on adipocyte differentiation. The inhibition of lipid accumulation was accompanied by a significant decrease in the expression levels of adipocyte molecular markers-peroxisome proliferator-activated receptor γ, CAAT enhancer binding protein α, and fatty acid-binding protein. Thus, this study determined that WPP EtOAc extract contains the inhibitory compound(s) on adipogenesis.

UR - http://www.scopus.com/inward/record.url?scp=61349093574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61349093574&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:61349093574

VL - 17

SP - 1327

EP - 1331

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

SN - 1226-7708

IS - 6

ER -