Inhibitory effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) in dermal fibroblast cells

Jae Seok Shim, Yi Young Kwon, Young Sun Han, Jae Kwan Hwang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Exposure of the skin to ultraviolet (UV) induces photoaging associated with up-regulated matrix metalloproteinases (MMPs) activities and decreased collagen synthesis. We previously reported that panduratin A, a chalcone compound isolated from Kaempferia pandurata Roxb., decreased MMP-1 expression in UV-irradiated human skin fibroblasts. Here, we have investigated the effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) signaling modules such as extracellular-regulated protein kinase (ERK), Jun-N-terminal kinase (JNK) and p38 kinase. Treatment with panduratin A in the range of 0.001-0.1 μM significantly inhibited UV-induced ERK, JNK and p38 activation. Moreover, inhibition of ERK, JNK and p38 by panduratin A resulted in decreased c-Fos expression and c-Jun phosphorylation induced by UV, which led to inhibition of activator protein-1 (AP-1) DNA binding activity. Panduratin A showed stronger activity than epigallocatechin 3-O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can down-regulate UV-induced MMP-1 expression by inhibiting the MAPKs pathways and AP-1 activation.

Original languageEnglish
Pages (from-to)1446-1450
Number of pages5
JournalPlanta Medica
Volume74
Issue number12
DOIs
Publication statusPublished - 2008 Oct 1

Fingerprint

Fibroblasts
Mitogen-Activated Protein Kinases
Chemical activation
Cells
Skin
Phosphotransferases
Protein Kinases
Matrix Metalloproteinase 1
Transcription Factor AP-1
Zingiberaceae
Chalcone
Phosphorylation
Matrix Metalloproteinases
panduratin A
Collagen
Down-Regulation
Aging of materials
DNA
protein kinase N

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

@article{2cf33b3224294fcfa0831836d3a3ff33,
title = "Inhibitory effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) in dermal fibroblast cells",
abstract = "Exposure of the skin to ultraviolet (UV) induces photoaging associated with up-regulated matrix metalloproteinases (MMPs) activities and decreased collagen synthesis. We previously reported that panduratin A, a chalcone compound isolated from Kaempferia pandurata Roxb., decreased MMP-1 expression in UV-irradiated human skin fibroblasts. Here, we have investigated the effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) signaling modules such as extracellular-regulated protein kinase (ERK), Jun-N-terminal kinase (JNK) and p38 kinase. Treatment with panduratin A in the range of 0.001-0.1 μM significantly inhibited UV-induced ERK, JNK and p38 activation. Moreover, inhibition of ERK, JNK and p38 by panduratin A resulted in decreased c-Fos expression and c-Jun phosphorylation induced by UV, which led to inhibition of activator protein-1 (AP-1) DNA binding activity. Panduratin A showed stronger activity than epigallocatechin 3-O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can down-regulate UV-induced MMP-1 expression by inhibiting the MAPKs pathways and AP-1 activation.",
author = "Shim, {Jae Seok} and Kwon, {Yi Young} and Han, {Young Sun} and Hwang, {Jae Kwan}",
year = "2008",
month = "10",
day = "1",
doi = "10.1055/s-2008-1081352",
language = "English",
volume = "74",
pages = "1446--1450",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "Georg Thieme Verlag",
number = "12",

}

Inhibitory effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) in dermal fibroblast cells. / Shim, Jae Seok; Kwon, Yi Young; Han, Young Sun; Hwang, Jae Kwan.

In: Planta Medica, Vol. 74, No. 12, 01.10.2008, p. 1446-1450.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibitory effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) in dermal fibroblast cells

AU - Shim, Jae Seok

AU - Kwon, Yi Young

AU - Han, Young Sun

AU - Hwang, Jae Kwan

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Exposure of the skin to ultraviolet (UV) induces photoaging associated with up-regulated matrix metalloproteinases (MMPs) activities and decreased collagen synthesis. We previously reported that panduratin A, a chalcone compound isolated from Kaempferia pandurata Roxb., decreased MMP-1 expression in UV-irradiated human skin fibroblasts. Here, we have investigated the effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) signaling modules such as extracellular-regulated protein kinase (ERK), Jun-N-terminal kinase (JNK) and p38 kinase. Treatment with panduratin A in the range of 0.001-0.1 μM significantly inhibited UV-induced ERK, JNK and p38 activation. Moreover, inhibition of ERK, JNK and p38 by panduratin A resulted in decreased c-Fos expression and c-Jun phosphorylation induced by UV, which led to inhibition of activator protein-1 (AP-1) DNA binding activity. Panduratin A showed stronger activity than epigallocatechin 3-O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can down-regulate UV-induced MMP-1 expression by inhibiting the MAPKs pathways and AP-1 activation.

AB - Exposure of the skin to ultraviolet (UV) induces photoaging associated with up-regulated matrix metalloproteinases (MMPs) activities and decreased collagen synthesis. We previously reported that panduratin A, a chalcone compound isolated from Kaempferia pandurata Roxb., decreased MMP-1 expression in UV-irradiated human skin fibroblasts. Here, we have investigated the effect of panduratin A on UV-induced activation of mitogen-activated protein kinases (MAPKs) signaling modules such as extracellular-regulated protein kinase (ERK), Jun-N-terminal kinase (JNK) and p38 kinase. Treatment with panduratin A in the range of 0.001-0.1 μM significantly inhibited UV-induced ERK, JNK and p38 activation. Moreover, inhibition of ERK, JNK and p38 by panduratin A resulted in decreased c-Fos expression and c-Jun phosphorylation induced by UV, which led to inhibition of activator protein-1 (AP-1) DNA binding activity. Panduratin A showed stronger activity than epigallocatechin 3-O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can down-regulate UV-induced MMP-1 expression by inhibiting the MAPKs pathways and AP-1 activation.

UR - http://www.scopus.com/inward/record.url?scp=54249083259&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54249083259&partnerID=8YFLogxK

U2 - 10.1055/s-2008-1081352

DO - 10.1055/s-2008-1081352

M3 - Article

C2 - 18683126

AN - SCOPUS:54249083259

VL - 74

SP - 1446

EP - 1450

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 12

ER -