Inhibitory effect of salmosin, a Korean snake venom-derived disintegrin, on the integrin αv-mediated proliferation of SK-Mel-2 human melanoma cells

Kwang Hoe Chung, Sung Hoon Kim, Kyu Yeon Han, Young Doug Sohn, Soo Ik Chang, Kwang Hyun Baek, Yangsoo Jang, Doo Sik Kim, In Cheol Kang

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28 Citations (Scopus)


We have investigated the inhibitory effect of salmosin on integrin-mediated human tumour cell proliferation. SK-Mel-2 human melanoma cell adhesion to denatured collagen or vitronectin was found to be significantly and statistically inhibited by salmosin in a dose-dependent manner (P<0.05). Moreover, the binding of SK-Mel-2 cells to salmosin-coated plates was specifically disrupted by anti-integrin αv monoclonal antibody at 8 μg mL-1, but not by anti-integrin monoclonal antibody. These findings indicated that salmosin inhibited the adhesion of SK-Mel-2 cells to denatured collagen by specifically blocking integrin αv. The proliferation of SK-Mel-2 cells on a denatured collagen-coated plate was statistically and significantly inhibited by salmosin induced apoptosis in a dose-dependent manner (P<0.05). Anti-integrin αv monoclonal antibody, anti-integrin αvβ3 monoclonal antibody, and synthetic RGD peptide also suppressed SK-Mel-2 cell proliferation. Several lines of experimental evidence strongly suggested that the inhibition of SK-Mel-2 cell proliferation by salmosin was due to the induction of apoptosis via the blocking of integrin αv-mediated cell survival.

Original languageEnglish
Pages (from-to)1577-1582
Number of pages6
JournalJournal of Pharmacy and Pharmacology
Issue number11
Publication statusPublished - 2003 Nov

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science


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