Inhibitory effect of salmosin, a Korean snake venom-derived disintegrin, on the integrin αv-mediated proliferation of SK-Mel-2 human melanoma cells

Kwang Hoe Chung, Sung Hoon Kim, Kyu Yeon Han, Young Doug Sohn, Soo Ik Chang, Kwang Hyun Baek, Yangsoo Jang, Doo Sik Kim, In Cheol Kang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have investigated the inhibitory effect of salmosin on integrin-mediated human tumour cell proliferation. SK-Mel-2 human melanoma cell adhesion to denatured collagen or vitronectin was found to be significantly and statistically inhibited by salmosin in a dose-dependent manner (P<0.05). Moreover, the binding of SK-Mel-2 cells to salmosin-coated plates was specifically disrupted by anti-integrin αv monoclonal antibody at 8 μg mL-1, but not by anti-integrin monoclonal antibody. These findings indicated that salmosin inhibited the adhesion of SK-Mel-2 cells to denatured collagen by specifically blocking integrin αv. The proliferation of SK-Mel-2 cells on a denatured collagen-coated plate was statistically and significantly inhibited by salmosin induced apoptosis in a dose-dependent manner (P<0.05). Anti-integrin αv monoclonal antibody, anti-integrin αvβ3 monoclonal antibody, and synthetic RGD peptide also suppressed SK-Mel-2 cell proliferation. Several lines of experimental evidence strongly suggested that the inhibition of SK-Mel-2 cell proliferation by salmosin was due to the induction of apoptosis via the blocking of integrin αv-mediated cell survival.

Original languageEnglish
Pages (from-to)1577-1582
Number of pages6
JournalJournal of Pharmacy and Pharmacology
Volume55
Issue number11
DOIs
Publication statusPublished - 2003 Nov 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this