Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

Phan Van Kiem, Chau Van Minh, Nguyen Xuan Nhiem, Nguyen Xuan Cuong, Bui Huu Tai, Tran Hong Quang, Hoang Le Tuan Anh, Pham Hai Yen, Ninh Khac Ban, Seung Hyun Kim, Mingjie Xin, Ji Yun Cha, Young Mi Lee, Young Ho Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.

Original languageEnglish
Pages (from-to)2135-2142
Number of pages8
JournalArchives of pharmacal research
Volume35
Issue number12
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Ficus
HT29 Cells
Interleukin-8
Galactose
Galactolipids
Cells
Cell Line
Anti-Inflammatory Agents
Bioassay
Fractionation
Biological Assay
Methanol
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

Cite this

Van Kiem, Phan ; Van Minh, Chau ; Nhiem, Nguyen Xuan ; Cuong, Nguyen Xuan ; Tai, Bui Huu ; Quang, Tran Hong ; Anh, Hoang Le Tuan ; Yen, Pham Hai ; Ban, Ninh Khac ; Kim, Seung Hyun ; Xin, Mingjie ; Cha, Ji Yun ; Lee, Young Mi ; Kim, Young Ho. / Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa. In: Archives of pharmacal research. 2012 ; Vol. 35, No. 12. pp. 2135-2142.
@article{3a722175bb1b47c0aa0a978275163b6f,
title = "Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa",
abstract = "Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40{\%} at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.",
author = "{Van Kiem}, Phan and {Van Minh}, Chau and Nhiem, {Nguyen Xuan} and Cuong, {Nguyen Xuan} and Tai, {Bui Huu} and Quang, {Tran Hong} and Anh, {Hoang Le Tuan} and Yen, {Pham Hai} and Ban, {Ninh Khac} and Kim, {Seung Hyun} and Mingjie Xin and Cha, {Ji Yun} and Lee, {Young Mi} and Kim, {Young Ho}",
year = "2012",
month = "12",
day = "1",
doi = "10.1007/s12272-012-1210-8",
language = "English",
volume = "35",
pages = "2135--2142",
journal = "Archives of Pharmacal Research",
issn = "0253-6269",
publisher = "Pharmaceutical Society of Korea",
number = "12",

}

Van Kiem, P, Van Minh, C, Nhiem, NX, Cuong, NX, Tai, BH, Quang, TH, Anh, HLT, Yen, PH, Ban, NK, Kim, SH, Xin, M, Cha, JY, Lee, YM & Kim, YH 2012, 'Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa', Archives of pharmacal research, vol. 35, no. 12, pp. 2135-2142. https://doi.org/10.1007/s12272-012-1210-8

Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa. / Van Kiem, Phan; Van Minh, Chau; Nhiem, Nguyen Xuan; Cuong, Nguyen Xuan; Tai, Bui Huu; Quang, Tran Hong; Anh, Hoang Le Tuan; Yen, Pham Hai; Ban, Ninh Khac; Kim, Seung Hyun; Xin, Mingjie; Cha, Ji Yun; Lee, Young Mi; Kim, Young Ho.

In: Archives of pharmacal research, Vol. 35, No. 12, 01.12.2012, p. 2135-2142.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

AU - Van Kiem, Phan

AU - Van Minh, Chau

AU - Nhiem, Nguyen Xuan

AU - Cuong, Nguyen Xuan

AU - Tai, Bui Huu

AU - Quang, Tran Hong

AU - Anh, Hoang Le Tuan

AU - Yen, Pham Hai

AU - Ban, Ninh Khac

AU - Kim, Seung Hyun

AU - Xin, Mingjie

AU - Cha, Ji Yun

AU - Lee, Young Mi

AU - Kim, Young Ho

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.

AB - Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.

UR - http://www.scopus.com/inward/record.url?scp=84875705460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875705460&partnerID=8YFLogxK

U2 - 10.1007/s12272-012-1210-8

DO - 10.1007/s12272-012-1210-8

M3 - Article

VL - 35

SP - 2135

EP - 2142

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

SN - 0253-6269

IS - 12

ER -