Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

Phan Van Kiem, Chau Van Minh, Nguyen Xuan Nhiem, Nguyen Xuan Cuong, Bui Huu Tai, Tran Hong Quang, Hoang Le Tuan Anh, Pham Hai Yen, Ninh Khac Ban, Seung Hyun Kim, Mingjie Xin, Ji Yun Cha, Young Mi Lee, Young Ho Kim

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13 Citations (Scopus)

Abstract

Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.

Original languageEnglish
Pages (from-to)2135-2142
Number of pages8
JournalArchives of pharmacal research
Volume35
Issue number12
DOIs
Publication statusPublished - 2012 Dec

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

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    Van Kiem, P., Van Minh, C., Nhiem, N. X., Cuong, N. X., Tai, B. H., Quang, T. H., Anh, H. L. T., Yen, P. H., Ban, N. K., Kim, S. H., Xin, M., Cha, J. Y., Lee, Y. M., & Kim, Y. H. (2012). Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa. Archives of pharmacal research, 35(12), 2135-2142. https://doi.org/10.1007/s12272-012-1210-8