Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

Phan Van Kiem; Chau Van Minh; Nguyen Xuan Nhiem; Nguyen Xuan Cuong; Bui Huu Tai; Tran Hong Quang; Hoang Le Tuan Anh; Pham Hai Yen; Ninh Khac Ban; Seung Hyun Kim; Mingjie Xin; Ji Yun Cha; Young Mi Lee; Young Ho Kim

doi:10.1007/s12272-012-1210-8

Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

Phan Van Kiem, Chau Van Minh, Nguyen Xuan Nhiem, Nguyen Xuan Cuong, Bui Huu Tai, Tran Hong Quang, Hoang Le Tuan Anh, Pham Hai Yen, Ninh Khac Ban, Seung Hyun Kim, Mingjie Xin, Ji Yun Cha, Young Mi Lee, Young Ho Kim

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.

Original language	English
Pages (from-to)	2135-2142
Number of pages	8
Journal	Archives of pharmacal research
Volume	35
Issue number	12
DOIs	https://doi.org/10.1007/s12272-012-1210-8
Publication status	Published - 2012 Dec

Bibliographical note

Funding Information:
This work was financially supported by Vietnam National Foundation for Science & Technology Development (Project No: 104.01.31.09) and Priority Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0093815), Republic of Korea.

All Science Journal Classification (ASJC) codes

Molecular Medicine
Drug Discovery
Organic Chemistry

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10.1007/s12272-012-1210-8

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Van Kiem, P., Van Minh, C., Nhiem, N. X., Cuong, N. X., Tai, B. H., Quang, T. H., Anh, H. L. T., Yen, P. H., Ban, N. K., Kim, S. H., Xin, M., Cha, J. Y., Lee, Y. M., & Kim, Y. H. (2012). Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa. Archives of pharmacal research, 35(12), 2135-2142. https://doi.org/10.1007/s12272-012-1210-8

@article{3a722175bb1b47c0aa0a978275163b6f,

title = "Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa",

abstract = "Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.",

author = "{Van Kiem}, Phan and {Van Minh}, Chau and Nhiem, {Nguyen Xuan} and Cuong, {Nguyen Xuan} and Tai, {Bui Huu} and Quang, {Tran Hong} and Anh, {Hoang Le Tuan} and Yen, {Pham Hai} and Ban, {Ninh Khac} and Kim, {Seung Hyun} and Mingjie Xin and Cha, {Ji Yun} and Lee, {Young Mi} and Kim, {Young Ho}",

note = "Funding Information: This work was financially supported by Vietnam National Foundation for Science & Technology Development (Project No: 104.01.31.09) and Priority Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0093815), Republic of Korea.",

year = "2012",

month = dec,

doi = "10.1007/s12272-012-1210-8",

language = "English",

volume = "35",

pages = "2135--2142",

journal = "Archives of Pharmacal Research",

issn = "0253-6269",

publisher = "Pharmaceutical Society of Korea",

number = "12",

}

Van Kiem, P, Van Minh, C, Nhiem, NX, Cuong, NX, Tai, BH, Quang, TH, Anh, HLT, Yen, PH, Ban, NK, Kim, SH, Xin, M, Cha, JY, Lee, YM & Kim, YH 2012, 'Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa', Archives of pharmacal research, vol. 35, no. 12, pp. 2135-2142. https://doi.org/10.1007/s12272-012-1210-8

TY - JOUR

T1 - Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

AU - Van Kiem, Phan

AU - Van Minh, Chau

AU - Nhiem, Nguyen Xuan

AU - Cuong, Nguyen Xuan

AU - Tai, Bui Huu

AU - Quang, Tran Hong

AU - Anh, Hoang Le Tuan

AU - Yen, Pham Hai

AU - Ban, Ninh Khac

AU - Kim, Seung Hyun

AU - Xin, Mingjie

AU - Cha, Ji Yun

AU - Lee, Young Mi

AU - Kim, Young Ho

N1 - Funding Information: This work was financially supported by Vietnam National Foundation for Science & Technology Development (Project No: 104.01.31.09) and Priority Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0093815), Republic of Korea.

PY - 2012/12

Y1 - 2012/12

N2 - Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.

AB - Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D- galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O- β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O- β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl- 6′-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6′-O-(α-D- galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.

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JO - Archives of Pharmacal Research

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ER -

Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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