Inhibitory effects of anthocyanins on secretion of helicobacter pylori CagA and VacA toxins

Sa Hyun Kim, Min Park, Hyunjun Woo, Nagendran Tharmalingam, Gyusang Lee, Kijong Rhee, Yong Bin Eom, Sang Ik Han, Woo Duck Seo, Jong Bae Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Anthocyanins have been studied as potential antimicrobial agents against Helicobacter pylori. We investigated whether the biosynthesis and secretion of cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) could be suppressed by anthocyanin treatment in vitro. H. pylori reference strain 60190 (CagA+/VacA+) was used in this study to investigate the inhibitory effects of anthocyanins; cyanidin 3-O-glucoside (C3G), peonidin 3-O-glucoside (Peo3G), pelargonidin 3-O-glucoside (Pel3G), and malvidin 3-O-glucoside (M3G) on expression and secretion of H. pylori toxins. Anthocyanins were added to bacterial cultures and Western blotting was used to determine secretion of CagA and VacA. Among them, we found that C3G inhibited secretion of CagA and VacA resulting in intracellular accumulation of CagA and VacA. C3G had no effect on cagA and vacA expression but suppressed secA transcription. As SecA is involved in translocation of bacterial proteins, the down-regulation of secA expression by C3G offers a mechanistic explanation for the inhibition of toxin secretion. To our knowledge, this is the first report suggesting that C3G inhibits secretion of the H. pylori toxins CagA and VacA via suppression of secA transcription.

Original languageEnglish
Pages (from-to)838-842
Number of pages5
JournalInternational Journal of Medical Sciences
Volume9
Issue number10
DOIs
Publication statusPublished - 2012 Nov 1

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Anthocyanins
Cytotoxins
Helicobacter pylori
Bacterial Proteins
Staphylococcal Protein A
Anti-Infective Agents
Down-Regulation
Western Blotting
cyanidin 3-O-glucoside

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Kim, Sa Hyun ; Park, Min ; Woo, Hyunjun ; Tharmalingam, Nagendran ; Lee, Gyusang ; Rhee, Kijong ; Eom, Yong Bin ; Han, Sang Ik ; Seo, Woo Duck ; Kim, Jong Bae. / Inhibitory effects of anthocyanins on secretion of helicobacter pylori CagA and VacA toxins. In: International Journal of Medical Sciences. 2012 ; Vol. 9, No. 10. pp. 838-842.
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abstract = "Anthocyanins have been studied as potential antimicrobial agents against Helicobacter pylori. We investigated whether the biosynthesis and secretion of cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) could be suppressed by anthocyanin treatment in vitro. H. pylori reference strain 60190 (CagA+/VacA+) was used in this study to investigate the inhibitory effects of anthocyanins; cyanidin 3-O-glucoside (C3G), peonidin 3-O-glucoside (Peo3G), pelargonidin 3-O-glucoside (Pel3G), and malvidin 3-O-glucoside (M3G) on expression and secretion of H. pylori toxins. Anthocyanins were added to bacterial cultures and Western blotting was used to determine secretion of CagA and VacA. Among them, we found that C3G inhibited secretion of CagA and VacA resulting in intracellular accumulation of CagA and VacA. C3G had no effect on cagA and vacA expression but suppressed secA transcription. As SecA is involved in translocation of bacterial proteins, the down-regulation of secA expression by C3G offers a mechanistic explanation for the inhibition of toxin secretion. To our knowledge, this is the first report suggesting that C3G inhibits secretion of the H. pylori toxins CagA and VacA via suppression of secA transcription.",
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Kim, SH, Park, M, Woo, H, Tharmalingam, N, Lee, G, Rhee, K, Eom, YB, Han, SI, Seo, WD & Kim, JB 2012, 'Inhibitory effects of anthocyanins on secretion of helicobacter pylori CagA and VacA toxins', International Journal of Medical Sciences, vol. 9, no. 10, pp. 838-842. https://doi.org/10.7150/ijms.5094

Inhibitory effects of anthocyanins on secretion of helicobacter pylori CagA and VacA toxins. / Kim, Sa Hyun; Park, Min; Woo, Hyunjun; Tharmalingam, Nagendran; Lee, Gyusang; Rhee, Kijong; Eom, Yong Bin; Han, Sang Ik; Seo, Woo Duck; Kim, Jong Bae.

In: International Journal of Medical Sciences, Vol. 9, No. 10, 01.11.2012, p. 838-842.

Research output: Contribution to journalArticle

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