Periodontal disease is triggered by the host immune response to pathogens in the microbial biofilm. Worsening of periodontal disease destroys the tooth-supporting tissues and alveolar bone. As oral inflammation can induce systemic diseases in humans, it is important to prevent periodontal disease. In this study, we demonstrated that Curcuma xanthorrhiza supercritical extract (CXS) and its active compound, xanthorrhizol (XAN), exhibit anti-inflammatory effects on lipopolysaccharide (LPS)-treated human gingival fibroblast-1 cells and anti-osteoclastic effects on receptor activator of nuclear factor kappa B ligand (RANKL)-treated RAW264.7 cells. LPS-upregulated inflammatory factors, such as nuclear factor kappa B p65 and interleukin-1β, were prominently reduced by CXS and XAN. In addition, RANKL-induced osteoclastic factors, such as nuclear factor of activated T-cells c1, tartrate-resistant acid phosphatase, and cathepsin K, were decreased in the presence of CXS and XAN. CXS and XAN inhibited the mitogen-activated protein kinase (MAPK)/activator protein-1 (AP-1) signaling pathway. Collectively, these results provide evidence that CXS and XAN suppress LPS-induced inflammation and RANKL-induced osteoclastogenesis by suppressing the MAPK/AP-1 pathway.
Bibliographical noteFunding Information:
This research was supported by the Yonsei University Future-leading Research Initiative of 2016 (RMS2 2016-11-1540) and partially by the World Class 300 Project R&D Program (S2435140) funded by the Small and Medium Business Administration (SMBA), Republic of Korea.
This research was supported by the Yonsei University Future-leading Research Initiative of 2016 (RMS2 2016-11- 1540) and partially by the World Class 300 Project R&D Program (S2435140) funded by the Small and Medium Business Administration (SMBA), Republic of Korea.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology