Inhibitory effects of panduratin A on allergy-related mediator production in rat basophilic leukemia mast cells

Yuri Choi, Myung Suk Kim, Jae Kwan Hwang

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20 Citations (Scopus)

Abstract

Immediate-type hypersensitivity is characterized by elevated levels of immunoglobulin E (IgE) and activated mast cell plays a crucial role by releasing granule contents, lipid-derived mediators, cytokines, and chemokines. To evaluate the antiallergic effects of panduratin A isolated from Boesenbergia pandurata Roxb., we determined its effects on calcium (Ca2+) influx, degranulation, and inflammatory mediators in calcium ionophore A23187 and phorbol 12-myristate 13-acetate (PMA)-stimulated rat basophilic leukemia (RBL-2H3) cells. Panduratin A (20 μM) inhibited secretion of β-hexosaminidase (46.69±9.6 %), histamine (34.32±2.1 %), and Ca2+ influx (43.84 %). Panduratin A reduced the production of prostaglandin E2 (PGE2, 47.58±3.4 %), leukotriene B4 (LTB4, 98.15±1.6 %), and the mRNA expression of cyclooxygenase-2, 5-lipoxygenase, interleukin (IL)-4, IL-13, and tumor necrosis factor-α. Furthermore, panduarin A attenuated phosphorylation of Akt, the mitogen-activated protein kinases (MAPK) extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) expression. These results indicate that panduratin A might be useful as an agent against immediate-type hypersensitivity.

Original languageEnglish
Pages (from-to)1904-1915
Number of pages12
JournalInflammation
Volume35
Issue number6
DOIs
Publication statusPublished - 2012 Dec 1

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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