Inhibitory effects of panduratin A on allergy-related mediator production in rat basophilic leukemia mast cells

Yuri Choi; Myung Suk Kim; Jae Kwan Hwang

doi:10.1007/s10753-012-9513-y

Inhibitory effects of panduratin A on allergy-related mediator production in rat basophilic leukemia mast cells

Yuri Choi, Myung Suk Kim, Jae Kwan Hwang

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Immediate-type hypersensitivity is characterized by elevated levels of immunoglobulin E (IgE) and activated mast cell plays a crucial role by releasing granule contents, lipid-derived mediators, cytokines, and chemokines. To evaluate the antiallergic effects of panduratin A isolated from Boesenbergia pandurata Roxb., we determined its effects on calcium (Ca²⁺) influx, degranulation, and inflammatory mediators in calcium ionophore A23187 and phorbol 12-myristate 13-acetate (PMA)-stimulated rat basophilic leukemia (RBL-2H3) cells. Panduratin A (20 μM) inhibited secretion of β-hexosaminidase (46.69±9.6 %), histamine (34.32±2.1 %), and Ca²⁺ influx (43.84 %). Panduratin A reduced the production of prostaglandin E₂ (PGE₂, 47.58±3.4 %), leukotriene B₄ (LTB₄, 98.15±1.6 %), and the mRNA expression of cyclooxygenase-2, 5-lipoxygenase, interleukin (IL)-4, IL-13, and tumor necrosis factor-α. Furthermore, panduarin A attenuated phosphorylation of Akt, the mitogen-activated protein kinases (MAPK) extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) expression. These results indicate that panduratin A might be useful as an agent against immediate-type hypersensitivity.

Original language	English
Pages (from-to)	1904-1915
Number of pages	12
Journal	Inflammation
Volume	35
Issue number	6
DOIs	https://doi.org/10.1007/s10753-012-9513-y
Publication status	Published - 2012 Dec

Bibliographical note

Funding Information:
This work was supported, in part, by the Yonsei Biomolecule Research Initiative of the Two-step Brain Korea 21 Project.

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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title = "Inhibitory effects of panduratin A on allergy-related mediator production in rat basophilic leukemia mast cells",

abstract = "Immediate-type hypersensitivity is characterized by elevated levels of immunoglobulin E (IgE) and activated mast cell plays a crucial role by releasing granule contents, lipid-derived mediators, cytokines, and chemokines. To evaluate the antiallergic effects of panduratin A isolated from Boesenbergia pandurata Roxb., we determined its effects on calcium (Ca2+) influx, degranulation, and inflammatory mediators in calcium ionophore A23187 and phorbol 12-myristate 13-acetate (PMA)-stimulated rat basophilic leukemia (RBL-2H3) cells. Panduratin A (20 μM) inhibited secretion of β-hexosaminidase (46.69±9.6 %), histamine (34.32±2.1 %), and Ca2+ influx (43.84 %). Panduratin A reduced the production of prostaglandin E2 (PGE2, 47.58±3.4 %), leukotriene B4 (LTB4, 98.15±1.6 %), and the mRNA expression of cyclooxygenase-2, 5-lipoxygenase, interleukin (IL)-4, IL-13, and tumor necrosis factor-α. Furthermore, panduarin A attenuated phosphorylation of Akt, the mitogen-activated protein kinases (MAPK) extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) expression. These results indicate that panduratin A might be useful as an agent against immediate-type hypersensitivity.",

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note = "Funding Information: This work was supported, in part, by the Yonsei Biomolecule Research Initiative of the Two-step Brain Korea 21 Project.",

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T1 - Inhibitory effects of panduratin A on allergy-related mediator production in rat basophilic leukemia mast cells

AU - Choi, Yuri

AU - Kim, Myung Suk

AU - Hwang, Jae Kwan

N1 - Funding Information: This work was supported, in part, by the Yonsei Biomolecule Research Initiative of the Two-step Brain Korea 21 Project.

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Inhibitory effects of panduratin A on allergy-related mediator production in rat basophilic leukemia mast cells

Abstract

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