Inhibitory mechanism of omega-3 fatty acids in pancreatic inflammation and apoptosis

Kyung Suk Park, Joo Weon Lim, Hye Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

38 Citations (Scopus)

Abstract

Oxidative stress is regarded as a major pathogenic factor in acute pancreatitis. Inflammation and apoptosis linked to oxidative stress has been implicated in cerulein-induced pancreatitis as an experimental model of acute pancreatitis. Recently, we found that reactive oxygen species mediate inflammatory cytokine expression and apoptosis of pancreatic acinar cells stimulated with cerulein. Omega-3 fatty acids show antioxidant action in various cells and tissues. In the present study, we investigated whether omega-3 fatty acids inhibit cytokine expression in cerulein-stimulated pancreatic acinar cells and whether omega-3 fatty acids suppress apoptotic cell death in pancreatic acinar cells exposed to hydrogen peroxide. We found that omega-3 fatty acids, such as docosahexaenoic acid (DHA) and α-linolenic acid (ALA), suppressed the expression of inflammatory cytokines (IL-1β, IL-6) and inhibited the activation of transcription factor activator protein-1 in cerulein-stimulated pancreatic acinar cells. DHA and ALA inhibited DNA fragmentation, inhibited the decrease in cell viability, and inhibited the expression of apoptotic genes (p53, Bax, apoptosis-inducing factor) induced by hydrogen peroxide in pancreatic acinar cells. In conclusion, omega-3 fatty acids may be beneficial for preventing oxidative stress-induced pancreatic inflammation and apoptosis by inhibiting inflammatory cytokine and apoptotic gene expression of pancreatic acinar cells.

Original languageEnglish
Title of host publicationNatural Compounds and Their Role in Apoptotic Cell Signaling Pathways
PublisherBlackwell Publishing Inc.
Pages421-427
Number of pages7
ISBN (Print)9781573317375
DOIs
Publication statusPublished - 2009 Jan 1

Publication series

NameAnnals of the New York Academy of Sciences
Volume1171
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Acinar Cells
Omega-3 Fatty Acids
Ceruletide
Oxidative stress
Apoptosis
Inflammation
Cytokines
alpha-Linolenic Acid
Pancreatitis
Docosahexaenoic Acids
Oxidative Stress
Hydrogen Peroxide
Apoptosis Inducing Factor
Transcription Factor AP-1
Gene Expression
Cell death
Interleukin-1
Gene expression
p53 Genes
DNA Fragmentation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Park, K. S., Lim, J. W., & Kim, H. Y. (2009). Inhibitory mechanism of omega-3 fatty acids in pancreatic inflammation and apoptosis. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways (pp. 421-427). (Annals of the New York Academy of Sciences; Vol. 1171). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04887.x
Park, Kyung Suk ; Lim, Joo Weon ; Kim, Hye Young. / Inhibitory mechanism of omega-3 fatty acids in pancreatic inflammation and apoptosis. Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. pp. 421-427 (Annals of the New York Academy of Sciences).
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Park, KS, Lim, JW & Kim, HY 2009, Inhibitory mechanism of omega-3 fatty acids in pancreatic inflammation and apoptosis. in Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Annals of the New York Academy of Sciences, vol. 1171, Blackwell Publishing Inc., pp. 421-427. https://doi.org/10.1111/j.1749-6632.2009.04887.x

Inhibitory mechanism of omega-3 fatty acids in pancreatic inflammation and apoptosis. / Park, Kyung Suk; Lim, Joo Weon; Kim, Hye Young.

Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. p. 421-427 (Annals of the New York Academy of Sciences; Vol. 1171).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Oxidative stress is regarded as a major pathogenic factor in acute pancreatitis. Inflammation and apoptosis linked to oxidative stress has been implicated in cerulein-induced pancreatitis as an experimental model of acute pancreatitis. Recently, we found that reactive oxygen species mediate inflammatory cytokine expression and apoptosis of pancreatic acinar cells stimulated with cerulein. Omega-3 fatty acids show antioxidant action in various cells and tissues. In the present study, we investigated whether omega-3 fatty acids inhibit cytokine expression in cerulein-stimulated pancreatic acinar cells and whether omega-3 fatty acids suppress apoptotic cell death in pancreatic acinar cells exposed to hydrogen peroxide. We found that omega-3 fatty acids, such as docosahexaenoic acid (DHA) and α-linolenic acid (ALA), suppressed the expression of inflammatory cytokines (IL-1β, IL-6) and inhibited the activation of transcription factor activator protein-1 in cerulein-stimulated pancreatic acinar cells. DHA and ALA inhibited DNA fragmentation, inhibited the decrease in cell viability, and inhibited the expression of apoptotic genes (p53, Bax, apoptosis-inducing factor) induced by hydrogen peroxide in pancreatic acinar cells. In conclusion, omega-3 fatty acids may be beneficial for preventing oxidative stress-induced pancreatic inflammation and apoptosis by inhibiting inflammatory cytokine and apoptotic gene expression of pancreatic acinar cells.

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Park KS, Lim JW, Kim HY. Inhibitory mechanism of omega-3 fatty acids in pancreatic inflammation and apoptosis. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc. 2009. p. 421-427. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.04887.x