Inkjet-based multilayered growth factor-releasing nanofilms for enhancing proliferation of mesenchymal stem cells in vitro

Moonhyun Choi, Daheui Choi, Uiyoung Han, Jinkee Hong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We report the preparation and characterization of inkjet-based basic fibroblast growth factor (bFGF)-containing nanofilms on a flexible PET substrate. bFGF and heparin (HEP) were assembled by inkjet-based layer-by-layer (LbL) assembly driven by electrostatic interactions. The bFGF/HEP nano-assembly surface coatings were formed via alternating printing adsorption of positively charged bFGF and negatively charged HEP; the process was monitored by UV–vis spectroscopy and quartz crystal microbalance. The bFGF release profile could be controlled by altering the number of layers of printed LbL films. Mesenchymal stem cells, which are capable of extended proliferation in vitro, require a continuous supply of bFGF for proliferation. However, enhancing mesenchymal stem cell proliferation by continuous supplying bFGF is difficult, even with medium replacement, because of the instability of bFGF. Here, we established a novel system for releasing bioactive bFGF from a modified surface by using an inkjet-based nanofilm fabrication method.

Original languageEnglish
Pages (from-to)36-40
Number of pages5
JournalJournal of Industrial and Engineering Chemistry
Volume50
DOIs
Publication statusPublished - 2017 Jun 25

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

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