Insertion of linear 8.4 μm diameter 16 channel carbon fiber electrode arrays for single unit recordings

Paras R. Patel, Kyounghwan Na, Huanan Zhang, Takashi D.Y. Kozai, Nicholas A. Kotov, Euisik Yoon, Cynthia A. Chestek

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Objective. Single carbon fiber electrodes (d = 8.4 μm) insulated with parylene-c and functionalized with PEDOT:pTS have been shown to record single unit activity but manual implantation of these devices with forceps can be difficult. Without an improvement in the insertion method any increase in the channel count by fabricating carbon fiber arrays would be impractical. In this study, we utilize a water soluble coating and structural backbones that allow us to create, implant, and record from fully functionalized arrays of carbon fibers with ∼150 μm pitch. Approach. Two approaches were tested for the insertion of carbon fiber arrays. The first method used a poly(ethylene glycol) (PEG) coating that temporarily stiffened the fibers while leaving a small portion at the tip exposed. The small exposed portion (500 μm-1 mm) readily penetrated the brain allowing for an insertion that did not require the handling of each fiber by forceps. The second method involved the fabrication of silicon support structures with individual shanks spaced 150 μm apart. Each shank consisted of a small groove that held an individual carbon fiber. Main results. Our results showed that the PEG coating allowed for the chronic implantation of carbon fiber arrays in five rats with unit activity detected at 31 days post-implant. The silicon support structures recorded single unit activity in three acute rat surgeries. In one of those surgeries a stacked device with three layers of silicon support structures and carbon fibers was built and shown to readily insert into the brain with unit activity on select sites. Significance. From these studies we have found that carbon fibers spaced at ∼150 μm readily insert into the brain. This greatly increases the recording density of chronic neural probes and paves the way for even higher density devices that have a minimal scarring response.

Original languageEnglish
Article number046009
JournalJournal of Neural Engineering
Volume12
Issue number4
DOIs
Publication statusPublished - 2015 Aug 1

Fingerprint

Carbon fibers
Electrodes
Silicon
Polyethylene glycols
Brain
Surgical Instruments
Equipment and Supplies
Coatings
Surgery
Rats
Ethylene Glycol
carbon fiber
Fibers
Cicatrix
Fabrication
Water

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

Cite this

Patel, Paras R. ; Na, Kyounghwan ; Zhang, Huanan ; Kozai, Takashi D.Y. ; Kotov, Nicholas A. ; Yoon, Euisik ; Chestek, Cynthia A. / Insertion of linear 8.4 μm diameter 16 channel carbon fiber electrode arrays for single unit recordings. In: Journal of Neural Engineering. 2015 ; Vol. 12, No. 4.
@article{40279406f8d94bfb99a3a7f2209ca412,
title = "Insertion of linear 8.4 μm diameter 16 channel carbon fiber electrode arrays for single unit recordings",
abstract = "Objective. Single carbon fiber electrodes (d = 8.4 μm) insulated with parylene-c and functionalized with PEDOT:pTS have been shown to record single unit activity but manual implantation of these devices with forceps can be difficult. Without an improvement in the insertion method any increase in the channel count by fabricating carbon fiber arrays would be impractical. In this study, we utilize a water soluble coating and structural backbones that allow us to create, implant, and record from fully functionalized arrays of carbon fibers with ∼150 μm pitch. Approach. Two approaches were tested for the insertion of carbon fiber arrays. The first method used a poly(ethylene glycol) (PEG) coating that temporarily stiffened the fibers while leaving a small portion at the tip exposed. The small exposed portion (500 μm-1 mm) readily penetrated the brain allowing for an insertion that did not require the handling of each fiber by forceps. The second method involved the fabrication of silicon support structures with individual shanks spaced 150 μm apart. Each shank consisted of a small groove that held an individual carbon fiber. Main results. Our results showed that the PEG coating allowed for the chronic implantation of carbon fiber arrays in five rats with unit activity detected at 31 days post-implant. The silicon support structures recorded single unit activity in three acute rat surgeries. In one of those surgeries a stacked device with three layers of silicon support structures and carbon fibers was built and shown to readily insert into the brain with unit activity on select sites. Significance. From these studies we have found that carbon fibers spaced at ∼150 μm readily insert into the brain. This greatly increases the recording density of chronic neural probes and paves the way for even higher density devices that have a minimal scarring response.",
author = "Patel, {Paras R.} and Kyounghwan Na and Huanan Zhang and Kozai, {Takashi D.Y.} and Kotov, {Nicholas A.} and Euisik Yoon and Chestek, {Cynthia A.}",
year = "2015",
month = "8",
day = "1",
doi = "10.1088/1741-2560/12/4/046009",
language = "English",
volume = "12",
journal = "Journal of Neural Engineering",
issn = "1741-2560",
publisher = "IOP Publishing Ltd.",
number = "4",

}

Insertion of linear 8.4 μm diameter 16 channel carbon fiber electrode arrays for single unit recordings. / Patel, Paras R.; Na, Kyounghwan; Zhang, Huanan; Kozai, Takashi D.Y.; Kotov, Nicholas A.; Yoon, Euisik; Chestek, Cynthia A.

In: Journal of Neural Engineering, Vol. 12, No. 4, 046009, 01.08.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Insertion of linear 8.4 μm diameter 16 channel carbon fiber electrode arrays for single unit recordings

AU - Patel, Paras R.

AU - Na, Kyounghwan

AU - Zhang, Huanan

AU - Kozai, Takashi D.Y.

AU - Kotov, Nicholas A.

AU - Yoon, Euisik

AU - Chestek, Cynthia A.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Objective. Single carbon fiber electrodes (d = 8.4 μm) insulated with parylene-c and functionalized with PEDOT:pTS have been shown to record single unit activity but manual implantation of these devices with forceps can be difficult. Without an improvement in the insertion method any increase in the channel count by fabricating carbon fiber arrays would be impractical. In this study, we utilize a water soluble coating and structural backbones that allow us to create, implant, and record from fully functionalized arrays of carbon fibers with ∼150 μm pitch. Approach. Two approaches were tested for the insertion of carbon fiber arrays. The first method used a poly(ethylene glycol) (PEG) coating that temporarily stiffened the fibers while leaving a small portion at the tip exposed. The small exposed portion (500 μm-1 mm) readily penetrated the brain allowing for an insertion that did not require the handling of each fiber by forceps. The second method involved the fabrication of silicon support structures with individual shanks spaced 150 μm apart. Each shank consisted of a small groove that held an individual carbon fiber. Main results. Our results showed that the PEG coating allowed for the chronic implantation of carbon fiber arrays in five rats with unit activity detected at 31 days post-implant. The silicon support structures recorded single unit activity in three acute rat surgeries. In one of those surgeries a stacked device with three layers of silicon support structures and carbon fibers was built and shown to readily insert into the brain with unit activity on select sites. Significance. From these studies we have found that carbon fibers spaced at ∼150 μm readily insert into the brain. This greatly increases the recording density of chronic neural probes and paves the way for even higher density devices that have a minimal scarring response.

AB - Objective. Single carbon fiber electrodes (d = 8.4 μm) insulated with parylene-c and functionalized with PEDOT:pTS have been shown to record single unit activity but manual implantation of these devices with forceps can be difficult. Without an improvement in the insertion method any increase in the channel count by fabricating carbon fiber arrays would be impractical. In this study, we utilize a water soluble coating and structural backbones that allow us to create, implant, and record from fully functionalized arrays of carbon fibers with ∼150 μm pitch. Approach. Two approaches were tested for the insertion of carbon fiber arrays. The first method used a poly(ethylene glycol) (PEG) coating that temporarily stiffened the fibers while leaving a small portion at the tip exposed. The small exposed portion (500 μm-1 mm) readily penetrated the brain allowing for an insertion that did not require the handling of each fiber by forceps. The second method involved the fabrication of silicon support structures with individual shanks spaced 150 μm apart. Each shank consisted of a small groove that held an individual carbon fiber. Main results. Our results showed that the PEG coating allowed for the chronic implantation of carbon fiber arrays in five rats with unit activity detected at 31 days post-implant. The silicon support structures recorded single unit activity in three acute rat surgeries. In one of those surgeries a stacked device with three layers of silicon support structures and carbon fibers was built and shown to readily insert into the brain with unit activity on select sites. Significance. From these studies we have found that carbon fibers spaced at ∼150 μm readily insert into the brain. This greatly increases the recording density of chronic neural probes and paves the way for even higher density devices that have a minimal scarring response.

UR - http://www.scopus.com/inward/record.url?scp=84937468228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937468228&partnerID=8YFLogxK

U2 - 10.1088/1741-2560/12/4/046009

DO - 10.1088/1741-2560/12/4/046009

M3 - Article

C2 - 26035638

AN - SCOPUS:84937468228

VL - 12

JO - Journal of Neural Engineering

JF - Journal of Neural Engineering

SN - 1741-2560

IS - 4

M1 - 046009

ER -