Insertion of Vertically Aligned Nanowires into Living Cells by Inkjet Printing of Cells

Donggyu Lee, Daehee Lee, Yulim Won, Hyeonaug Hong, Yongjae Kim, Hyunwoo Song, Jae Chul Pyun, Yong Soo Cho, Wonhyoung Ryu, Jooho Moon

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Effective insertion of vertically aligned nanowires (NWs) into cells is critical for bioelectrical and biochemical devices, biological delivery systems, and photosynthetic bioenergy harvesting. However, accurate insertion of NWs into living cells using scalable processes has not yet been achieved. Here, NWs are inserted into living Chlamydomonas reinhardtii cells (Chlamy cells) via inkjet printing of the Chlamy cells, representing a low-cost and large-scale method for inserting NWs into living cells. Jetting conditions and printable bioink composed of living Chlamy cells are optimized to achieve stable jetting and precise ink deposition of bioink for indentation of NWs into Chlamy cells. Fluorescence confocal microscopy is used to verify the viability of Chlamy cells after inkjet printing. Simple mechanical considerations of the cell membrane and droplet kinetics are developed to control the jetting force to allow penetration of the NWs into cells. The results suggest that inkjet printing is an effective, controllable tool for stable insertion of NWs into cells with economic and scale-related advantages.

Original languageEnglish
Pages (from-to)1446-1457
Number of pages12
Issue number11
Publication statusPublished - 2016 Mar 16

Bibliographical note

Funding Information:
D.L. and D.L. contributed equally to this work. The authors received fi nancial support from grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Nos. 2012R1A3A2026417 and 2011-002-0285).

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)


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