Insertion of Vertically Aligned Nanowires into Living Cells by Inkjet Printing of Cells

Donggyu Lee, Daehee Lee, Yulim Won, Hyeonaug Hong, Yongjae Kim, Hyunwoo Song, Jae Chul Pyun, Yong Soo Cho, WonHyoung Ryu, Joo Ho Moon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Effective insertion of vertically aligned nanowires (NWs) into cells is critical for bioelectrical and biochemical devices, biological delivery systems, and photosynthetic bioenergy harvesting. However, accurate insertion of NWs into living cells using scalable processes has not yet been achieved. Here, NWs are inserted into living Chlamydomonas reinhardtii cells (Chlamy cells) via inkjet printing of the Chlamy cells, representing a low-cost and large-scale method for inserting NWs into living cells. Jetting conditions and printable bioink composed of living Chlamy cells are optimized to achieve stable jetting and precise ink deposition of bioink for indentation of NWs into Chlamy cells. Fluorescence confocal microscopy is used to verify the viability of Chlamy cells after inkjet printing. Simple mechanical considerations of the cell membrane and droplet kinetics are developed to control the jetting force to allow penetration of the NWs into cells. The results suggest that inkjet printing is an effective, controllable tool for stable insertion of NWs into cells with economic and scale-related advantages.

Original languageEnglish
Pages (from-to)1446-1457
Number of pages12
JournalSmall
Volume12
Issue number11
DOIs
Publication statusPublished - 2016 Mar 16

Fingerprint

Nanowires
Printing
Cells
Chlamydomonas reinhardtii
Confocal microscopy
Fluorescence microscopy
Cell membranes
Indentation
Ink
Fluorescence Microscopy
Confocal Microscopy
Economics
Kinetics
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

Cite this

Lee, Donggyu ; Lee, Daehee ; Won, Yulim ; Hong, Hyeonaug ; Kim, Yongjae ; Song, Hyunwoo ; Pyun, Jae Chul ; Cho, Yong Soo ; Ryu, WonHyoung ; Moon, Joo Ho. / Insertion of Vertically Aligned Nanowires into Living Cells by Inkjet Printing of Cells. In: Small. 2016 ; Vol. 12, No. 11. pp. 1446-1457.
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Insertion of Vertically Aligned Nanowires into Living Cells by Inkjet Printing of Cells. / Lee, Donggyu; Lee, Daehee; Won, Yulim; Hong, Hyeonaug; Kim, Yongjae; Song, Hyunwoo; Pyun, Jae Chul; Cho, Yong Soo; Ryu, WonHyoung; Moon, Joo Ho.

In: Small, Vol. 12, No. 11, 16.03.2016, p. 1446-1457.

Research output: Contribution to journalArticle

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