InsP6-sensitive variants of the Gle1 mRNA export factor rescue growth and fertility defects of the ipk1 low-phytic-acid mutation in arabidopsis

Ho Seok Lee, Du Hwa Lee, Hui Kyung Cho, Song Hee Kim, Joong Hyuck Auh, Hyunsook Pai

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP6), also known as phytic acid, accumulates in large quantities in plant seeds, serving as a phosphorus reservoir, but is an animal antinutrient and an important source of water pollution. Here, we report that Gle1 (GLFG lethal 1) in conjunction with InsP6 functions as an activator of the ATPase/RNA helicase LOS4 (low expression of osmotically responsive genes 4), which is involved in mRNA export in plants, supporting the Gle1-InsP6-Dbp5 (LOS4 homolog) paradigm proposed in yeast. Interestingly, plant Gle1 proteins have modifications in several key residues of the InsP6 binding pocket, which reduce the basicity of the surface charge. Arabidopsis thaliana Gle1 variants containing mutations that increase the basic charge of the InsP6 binding surface show increased sensitivity to InsP6 concentrations for the stimulation of LOS4 ATPase activity in vitro. Expression of the Gle1 variants with enhanced InsP6 sensitivity rescues the mRNA export defect of the ipk1 (inositol 1,3,4,5,6-pentakisphosphate 2-kinase) InsP6-deficient mutant and, furthermore, significantly improves vegetative growth, seed yield, and seed performance of the mutant. These results suggest that Gle1 is an important factor responsible for mediating InsP6 functions in plant growth and reproduction and that Gle1 variants with increased InsP6 sensitivity may be useful for engineering high-yielding low-phytate crops.

Original languageEnglish
Pages (from-to)417-431
Number of pages15
JournalPlant Cell
Volume27
Issue number2
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Phytic Acid
phytic acid
lethal genes
Arabidopsis
Fertility
Intercellular Signaling Peptides and Proteins
mutation
Seeds
Messenger RNA
Mutation
Adenosine Triphosphatases
Activator Appliances
RNA Helicases
Water Pollution
Inositol
Growth
adenosinetriphosphatase
Phosphorus
Reproduction
RNA helicases

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Lee, Ho Seok ; Lee, Du Hwa ; Cho, Hui Kyung ; Kim, Song Hee ; Auh, Joong Hyuck ; Pai, Hyunsook. / InsP6-sensitive variants of the Gle1 mRNA export factor rescue growth and fertility defects of the ipk1 low-phytic-acid mutation in arabidopsis. In: Plant Cell. 2015 ; Vol. 27, No. 2. pp. 417-431.
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InsP6-sensitive variants of the Gle1 mRNA export factor rescue growth and fertility defects of the ipk1 low-phytic-acid mutation in arabidopsis. / Lee, Ho Seok; Lee, Du Hwa; Cho, Hui Kyung; Kim, Song Hee; Auh, Joong Hyuck; Pai, Hyunsook.

In: Plant Cell, Vol. 27, No. 2, 01.01.2015, p. 417-431.

Research output: Contribution to journalArticle

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