Insulin particles as building blocks for controlled insulin release multilayer nano-films

Xiangde Lin, Daheui Choi, Jinkee Hong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Insulin nanoparticles (NPs) were prepared by pH-shift precipitation and a newly developed disassembly method at room temperature. Then, an electrostatic interaction-based, layer-by-layer (LbL) multilayer film incorporating insulin NPs was fabricated with poly(acrylic acid) (PAA) and poly(allylamine hydrochloride) (PAH), which is described herein as Si/(PAH/PAA)5(PAH/PAA-insulin NPs)n. The positively charged insulin NPs were introduced into the LbL film in the form of biocompatible PAA-insulin NP aggregates at a pH of 4.5 and were released in phosphate-buffered saline (pH 7.4), triggered by changes in the charges of the insulin molecules. In addition, the insulin-incorporated multilayer was swollen because of the different ionic environment, leading also to insulin release. Eighty percent of the insulin was released from the LBL film in the first stage of 3 h, and sustained release could be maintained in the second stage for up to 7 days in vitro, which is very critical for specific diabetic patients. These striking findings could offer novel directions to researchers in establishing insulin delivery systems for diabetic therapy and fabricating other protein nanoparticles applied to various biomedical platforms.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalMaterials Science and Engineering C
Volume54
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

insulin
Insulin
Multilayers
Nanoparticles
nanoparticles
hydrochlorides
carbopol 940
Multilayer films
acrylic acid
Coulomb interactions
Static Electricity
Acrylics
therapy
delivery
phosphates
Phosphates
platforms
Research Personnel
electrostatics
proteins

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials
  • Medicine(all)

Cite this

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abstract = "Insulin nanoparticles (NPs) were prepared by pH-shift precipitation and a newly developed disassembly method at room temperature. Then, an electrostatic interaction-based, layer-by-layer (LbL) multilayer film incorporating insulin NPs was fabricated with poly(acrylic acid) (PAA) and poly(allylamine hydrochloride) (PAH), which is described herein as Si/(PAH/PAA)5(PAH/PAA-insulin NPs)n. The positively charged insulin NPs were introduced into the LbL film in the form of biocompatible PAA-insulin NP aggregates at a pH of 4.5 and were released in phosphate-buffered saline (pH 7.4), triggered by changes in the charges of the insulin molecules. In addition, the insulin-incorporated multilayer was swollen because of the different ionic environment, leading also to insulin release. Eighty percent of the insulin was released from the LBL film in the first stage of 3 h, and sustained release could be maintained in the second stage for up to 7 days in vitro, which is very critical for specific diabetic patients. These striking findings could offer novel directions to researchers in establishing insulin delivery systems for diabetic therapy and fabricating other protein nanoparticles applied to various biomedical platforms.",
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Insulin particles as building blocks for controlled insulin release multilayer nano-films. / Lin, Xiangde; Choi, Daheui; Hong, Jinkee.

In: Materials Science and Engineering C, Vol. 54, 01.09.2015, p. 239-244.

Research output: Contribution to journalArticle

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