Integrated 3D macro-trapping and light-sheet imaging system

Zhengyi Yang, Peeter Piksarv, David E.K. Ferrier, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation XII
EditorsGabriel C. Spalding, Kishan Dholakia, Kishan Dholakia, Gabriel C. Spalding
PublisherSPIE
ISBN (Electronic)9781628417142, 9781628417142
DOIs
Publication statusPublished - 2015 Jan 1
EventOptical Trapping and Optical Micromanipulation XII - San Diego, United States
Duration: 2015 Aug 92015 Aug 12

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume9548
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

OtherOptical Trapping and Optical Micromanipulation XII
CountryUnited States
CitySan Diego
Period15/8/915/8/12

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Yang, Z., Piksarv, P., Ferrier, D. E. K., Gunn-Moore, F. J., & Dholakia, K. (2015). Integrated 3D macro-trapping and light-sheet imaging system. In G. C. Spalding, K. Dholakia, K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation XII [95480T] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9548). SPIE. https://doi.org/10.1117/12.2186399