Integrated 3D macro-trapping and light-sheet imaging system

Zhengyi Yang, Peeter Piksarv, David E.K. Ferrier, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation XII
EditorsGabriel C. Spalding, Kishan Dholakia, Kishan Dholakia, Gabriel C. Spalding
PublisherSPIE
ISBN (Electronic)9781628417142, 9781628417142
DOIs
Publication statusPublished - 2015 Jan 1
EventOptical Trapping and Optical Micromanipulation XII - San Diego, United States
Duration: 2015 Aug 92015 Aug 12

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume9548
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

OtherOptical Trapping and Optical Micromanipulation XII
CountryUnited States
CitySan Diego
Period15/8/915/8/12

Fingerprint

Trapping
Imaging System
Imaging systems
Macros
trapping
Imaging
Imaging techniques
Damage
Optical Forces
Tobacco
damage
larvae
tobacco
Embryo
Microscopy
embryos
High Speed
imaging techniques
embedding
Sepharose

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Yang, Z., Piksarv, P., Ferrier, D. E. K., Gunn-Moore, F. J., & Dholakia, K. (2015). Integrated 3D macro-trapping and light-sheet imaging system. In G. C. Spalding, K. Dholakia, K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation XII [95480T] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9548). SPIE. https://doi.org/10.1117/12.2186399
Yang, Zhengyi ; Piksarv, Peeter ; Ferrier, David E.K. ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Integrated 3D macro-trapping and light-sheet imaging system. Optical Trapping and Optical Micromanipulation XII. editor / Gabriel C. Spalding ; Kishan Dholakia ; Kishan Dholakia ; Gabriel C. Spalding. SPIE, 2015. (Proceedings of SPIE - The International Society for Optical Engineering).
@inproceedings{0b05dc69de1f4c79b352518096dca090,
title = "Integrated 3D macro-trapping and light-sheet imaging system",
abstract = "Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.",
author = "Zhengyi Yang and Peeter Piksarv and Ferrier, {David E.K.} and Gunn-Moore, {Frank J.} and Kishan Dholakia",
year = "2015",
month = "1",
day = "1",
doi = "10.1117/12.2186399",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Spalding, {Gabriel C.} and Kishan Dholakia and Kishan Dholakia and Spalding, {Gabriel C.}",
booktitle = "Optical Trapping and Optical Micromanipulation XII",
address = "United States",

}

Yang, Z, Piksarv, P, Ferrier, DEK, Gunn-Moore, FJ & Dholakia, K 2015, Integrated 3D macro-trapping and light-sheet imaging system. in GC Spalding, K Dholakia, K Dholakia & GC Spalding (eds), Optical Trapping and Optical Micromanipulation XII., 95480T, Proceedings of SPIE - The International Society for Optical Engineering, vol. 9548, SPIE, Optical Trapping and Optical Micromanipulation XII, San Diego, United States, 15/8/9. https://doi.org/10.1117/12.2186399

Integrated 3D macro-trapping and light-sheet imaging system. / Yang, Zhengyi; Piksarv, Peeter; Ferrier, David E.K.; Gunn-Moore, Frank J.; Dholakia, Kishan.

Optical Trapping and Optical Micromanipulation XII. ed. / Gabriel C. Spalding; Kishan Dholakia; Kishan Dholakia; Gabriel C. Spalding. SPIE, 2015. 95480T (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9548).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Integrated 3D macro-trapping and light-sheet imaging system

AU - Yang, Zhengyi

AU - Piksarv, Peeter

AU - Ferrier, David E.K.

AU - Gunn-Moore, Frank J.

AU - Dholakia, Kishan

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

AB - Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

UR - http://www.scopus.com/inward/record.url?scp=84951089123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951089123&partnerID=8YFLogxK

U2 - 10.1117/12.2186399

DO - 10.1117/12.2186399

M3 - Conference contribution

AN - SCOPUS:84951089123

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Optical Trapping and Optical Micromanipulation XII

A2 - Spalding, Gabriel C.

A2 - Dholakia, Kishan

A2 - Dholakia, Kishan

A2 - Spalding, Gabriel C.

PB - SPIE

ER -

Yang Z, Piksarv P, Ferrier DEK, Gunn-Moore FJ, Dholakia K. Integrated 3D macro-trapping and light-sheet imaging system. In Spalding GC, Dholakia K, Dholakia K, Spalding GC, editors, Optical Trapping and Optical Micromanipulation XII. SPIE. 2015. 95480T. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.2186399