Integrated holographic system for all-optical manipulation of developing embryos

Maria Leilani Torres-Mapa, Maciej Antkowiak, Hana Cizmarova, David E.K. Ferrier, Kishan Dholakia, Frank J. Gunn-Moore

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.

Original languageEnglish
Pages (from-to)1564-1575
Number of pages12
JournalBiomedical Optics Express
Volume2
Issue number6
DOIs
Publication statusPublished - 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'Integrated holographic system for all-optical manipulation of developing embryos'. Together they form a unique fingerprint.

  • Cite this

    Torres-Mapa, M. L., Antkowiak, M., Cizmarova, H., Ferrier, D. E. K., Dholakia, K., & Gunn-Moore, F. J. (2011). Integrated holographic system for all-optical manipulation of developing embryos. Biomedical Optics Express, 2(6), 1564-1575. https://doi.org/10.1364/BOE.2.001564