Abstract
We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.
| Original language | English |
|---|---|
| Pages (from-to) | 1564-1575 |
| Number of pages | 12 |
| Journal | Biomedical Optics Express |
| Volume | 2 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2011 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Atomic and Molecular Physics, and Optics
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Integrated holographic system for all-optical manipulation of developing embryos. / Torres-Mapa, Maria Leilani; Antkowiak, Maciej; Cizmarova, Hana; Ferrier, David E.K.; Dholakia, Kishan; Gunn-Moore, Frank J.
In: Biomedical Optics Express, Vol. 2, No. 6, 01.01.2011, p. 1564-1575.Research output: Contribution to journal › Article
TY - JOUR
T1 - Integrated holographic system for all-optical manipulation of developing embryos
AU - Torres-Mapa, Maria Leilani
AU - Antkowiak, Maciej
AU - Cizmarova, Hana
AU - Ferrier, David E.K.
AU - Dholakia, Kishan
AU - Gunn-Moore, Frank J.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.
AB - We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.
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UR - http://www.scopus.com/inward/citedby.url?scp=84861920133&partnerID=8YFLogxK
U2 - 10.1364/BOE.2.001564
DO - 10.1364/BOE.2.001564
M3 - Article
C2 - 21698019
AN - SCOPUS:84861920133
VL - 2
SP - 1564
EP - 1575
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 6
ER -