Integrated holographic system for all-optical manipulation of developing embryos

Maria Leilani Torres-Mapa, Maciej Antkowiak, Hana Cizmarova, David E.K. Ferrier, Kishan Dholakia, Frank J. Gunn-Moore

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.

Original languageEnglish
Pages (from-to)1564-1575
Number of pages12
JournalBiomedical Optics Express
Volume2
Issue number6
DOIs
Publication statusPublished - 2011 Jan 1

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Optical Devices
embryos
manipulators
Embryonic Structures
Lasers
Aluminum Oxide
sapphire
cells
Optical Tweezers
lasers
Chorion
Developmental Biology
Blastomeres
beam steering
Cell Lineage
light modulators
multiplexing
biology
viability
continuous radiation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Torres-Mapa, M. L., Antkowiak, M., Cizmarova, H., Ferrier, D. E. K., Dholakia, K., & Gunn-Moore, F. J. (2011). Integrated holographic system for all-optical manipulation of developing embryos. Biomedical Optics Express, 2(6), 1564-1575. https://doi.org/10.1364/BOE.2.001564
Torres-Mapa, Maria Leilani ; Antkowiak, Maciej ; Cizmarova, Hana ; Ferrier, David E.K. ; Dholakia, Kishan ; Gunn-Moore, Frank J. / Integrated holographic system for all-optical manipulation of developing embryos. In: Biomedical Optics Express. 2011 ; Vol. 2, No. 6. pp. 1564-1575.
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Torres-Mapa, ML, Antkowiak, M, Cizmarova, H, Ferrier, DEK, Dholakia, K & Gunn-Moore, FJ 2011, 'Integrated holographic system for all-optical manipulation of developing embryos', Biomedical Optics Express, vol. 2, no. 6, pp. 1564-1575. https://doi.org/10.1364/BOE.2.001564

Integrated holographic system for all-optical manipulation of developing embryos. / Torres-Mapa, Maria Leilani; Antkowiak, Maciej; Cizmarova, Hana; Ferrier, David E.K.; Dholakia, Kishan; Gunn-Moore, Frank J.

In: Biomedical Optics Express, Vol. 2, No. 6, 01.01.2011, p. 1564-1575.

Research output: Contribution to journalArticle

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