Integrated single- and two-photon light sheet microscopy using accelerating beams

Peeter Piksarv, Dominik Marti, Tuan Le, Angelika Unterhuber, Lindsey H. Forbes, Melissa R. Andrews, Andreas Stingl, Wolfgang Drexler, Peter E. Andersen, Kishan Dholakia

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Abstract

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

Original languageEnglish
Article number1435
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Piksarv, P., Marti, D., Le, T., Unterhuber, A., Forbes, L. H., Andrews, M. R., Stingl, A., Drexler, W., Andersen, P. E., & Dholakia, K. (2017). Integrated single- and two-photon light sheet microscopy using accelerating beams. Scientific reports, 7(1), [1435]. https://doi.org/10.1038/s41598-017-01543-4