Integrated single- and two-photon light sheet microscopy using accelerating beams

Peeter Piksarv, Dominik Marti, Tuan Le, Angelika Unterhuber, Lindsey H. Forbes, Melissa R. Andrews, Andreas Stingl, Wolfgang Drexler, Peter E. Andersen, Kishan Dholakia

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

Original languageEnglish
Article number1435
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Photons
Microscopy
Light

All Science Journal Classification (ASJC) codes

  • General

Cite this

Piksarv, Peeter ; Marti, Dominik ; Le, Tuan ; Unterhuber, Angelika ; Forbes, Lindsey H. ; Andrews, Melissa R. ; Stingl, Andreas ; Drexler, Wolfgang ; Andersen, Peter E. ; Dholakia, Kishan. / Integrated single- and two-photon light sheet microscopy using accelerating beams. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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Piksarv, P, Marti, D, Le, T, Unterhuber, A, Forbes, LH, Andrews, MR, Stingl, A, Drexler, W, Andersen, PE & Dholakia, K 2017, 'Integrated single- and two-photon light sheet microscopy using accelerating beams', Scientific reports, vol. 7, no. 1, 1435. https://doi.org/10.1038/s41598-017-01543-4

Integrated single- and two-photon light sheet microscopy using accelerating beams. / Piksarv, Peeter; Marti, Dominik; Le, Tuan; Unterhuber, Angelika; Forbes, Lindsey H.; Andrews, Melissa R.; Stingl, Andreas; Drexler, Wolfgang; Andersen, Peter E.; Dholakia, Kishan.

In: Scientific reports, Vol. 7, No. 1, 1435, 01.12.2017.

Research output: Contribution to journalArticle

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Piksarv P, Marti D, Le T, Unterhuber A, Forbes LH, Andrews MR et al. Integrated single- and two-photon light sheet microscopy using accelerating beams. Scientific reports. 2017 Dec 1;7(1). 1435. https://doi.org/10.1038/s41598-017-01543-4